| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Dennison, Helen E (14)
|
NCECA |
2:20.22 |
2:21.72 |
1.50 |
| 2 |
Buscher, Tessa G (16)
|
NCMOR |
2:27.93 |
2:32.34 |
4.41 |
| 2 |
Holmes, Mary E (14)
|
NCNCAC |
2:28.57 |
2:32.34 |
3.77 |
| 4 |
Palmateer, Rebecca A (14)
|
NCGFSC |
2:38.07 |
2:39.42 |
1.35 |
| 5 |
Hickey, Regan E (15)
|
NCMOR |
2:42.63 |
2:39.54 |
-3.09 |
| 6 |
Kim, Riley S (13)
|
NCNCAC |
2:40.14 |
2:41.76 |
1.62 |
| 7 |
Johnson, Addison L (13)
|
NCRSA |
2:48.24 |
2:43.01 |
-5.23 |
| 8 |
Loes, Emily C (13)
|
NCFISH |
2:52.74 |
2:43.78 |
-8.96 |
| 9 |
Aqrabawi, Rokaya A (14)
|
NCMOR |
2:51.42 |
2:45.61 |
-5.81 |
| 10 |
MacKinnon, Sarah M (13)
|
NCRSA |
2:48.10 |
2:48.24 |
0.14 |
| 11 |
Chafalovitch, Katrina F (13)
|
NCMOR |
2:55.00 |
2:55.03 |
0.03 |
| 12 |
Adkins, Kate H (13)
|
NCRSA |
|
3:40.08 |
- |
|
Epstein, Rebecca G (14)
|
NCMOR |
2:36.31 |
NS |
- |
|
Care, Megan E (16)
|
NCVACB |
2:54.59 |
NS |
- |