| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Cantin, Logan J (15)
|
VASRVA |
28.82 |
24.63 |
-4.19 |
| 2 |
Thornberry, Joseph J (16)
|
VAUN-S |
26.43 |
25.28 |
-1.15 |
| 3 |
Gravitt, Aidan S (16)
|
VASRVA |
25.31 |
25.77 |
0.46 |
| 4 |
Athey-Strayer, Shane M (18)
|
VANOVA |
25.40 |
26.10 |
0.70 |
| 5 |
Hollinshead, Jonathan A (16)
|
VASRVA |
|
27.08 |
- |
| 6 |
DeVries, Reese R (17)
|
VASRVA |
26.34 |
27.10 |
0.76 |
| 7 |
Hintze, Kai E (16)
|
VANOVA |
28.17 |
27.80 |
-0.37 |
| 8 |
Taylor, James D (15)
|
VANOVA |
28.29 |
28.50 |
0.21 |
| 9 |
Wharton, Collin S (15)
|
VASRVA |
|
32.84 |
- |
|
Zhao, Anran (16)
|
VANOVA |
27.75 |
NS |
- |
|
Thurber, Owen E (16)
|
VASRVA |
|
NS |
- |