| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Cabral, Carlton (10)
|
NIRRST |
|
49.79 |
- |
| 2 |
Stahl, Reed P (10)
|
NIVICT |
49.55 |
51.45 |
1.90 |
| 3 |
Schmidt, Million K (10)
|
NIRRST |
52.33 |
51.59 |
-0.74 |
| 4 |
Shields, Zachary W (10)
|
NIVICT |
52.42 |
51.62 |
-0.80 |
| 5 |
Stancampiano, Neel M (10)
|
NIPACK |
55.59 |
53.03 |
-2.56 |
| 6 |
Dinh, Brendan D (10)
|
NIWEBS |
53.18 |
53.66 |
0.48 |
| 7 |
He, Andrew (10)
|
NIVICT |
57.14 |
55.25 |
-1.89 |
| 8 |
Cottone, Cameron A (10)
|
NIGCOM |
57.16 |
55.61 |
-1.55 |
| 9 |
Ray, Gavin M (10)
|
NIVICT |
1:17.33 |
57.00 |
-20.33 |
| 10 |
Bajus, Nathan J (10)
|
NIVICT |
55.68 |
57.51 |
1.83 |
| 11 |
Blatt, Quinlan J (10)
|
NISDSC |
1:22.05 |
1:01.77 |
-20.28 |
| 12 |
Pogroszewski, Noah Z (10)
|
NIGCOM |
|
1:03.80 |
- |
| 13 |
Panfil, Tyler J (10)
|
NIGRSC |
1:01.77 |
1:05.22 |
3.45 |
| 14 |
Richards-Dyson, Jax E (10)
|
NIGCOM |
1:01.51 |
1:06.36 |
4.85 |
| 15 |
Wojieck, Mason G (10)
|
NIWGSC |
1:04.51 |
1:08.69 |
4.18 |
|
Madison, Benjamin M (10)
|
NIWEBS |
1:09.84 |
NS |
- |