| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Stahl, Reed P (10)
|
NIVICT |
47.30 |
44.65 |
-2.65 |
| 2 |
Cabral, Carlton (10)
|
NIRRST |
|
44.75 |
- |
| 3 |
Stancampiano, Neel M (10)
|
NIPACK |
|
45.23 |
- |
| 4 |
Gonzalez, Charles M (10)
|
NIPACK |
44.77 |
46.06 |
1.29 |
| 5 |
Panfil, Tyler J (10)
|
NIGRSC |
46.64 |
46.22 |
-0.42 |
| 6 |
Schmidt, Millenium K (10)
|
NIRRST |
|
46.45 |
- |
| 7 |
Kendall, Logan F (10)
|
NIMERC |
45.41 |
46.68 |
1.27 |
| 8 |
Ramsperger, Jacob T (10)
|
NISDSC |
50.47 |
47.18 |
-3.29 |
| 9 |
Richards-Dyson, Jax E (10)
|
NIGCOM |
47.55 |
48.40 |
0.85 |
| 10 |
Shields, Zachary W (10)
|
NIVICT |
46.28 |
48.48 |
2.20 |
| 11 |
Madison, Benjamin M (10)
|
NIWEBS |
54.97 |
48.98 |
-5.99 |
| 12 |
Schmidt, Million K (10)
|
NIRRST |
|
49.63 |
- |
| 13 |
Ray, Gavin M (10)
|
NIVICT |
46.61 |
50.19 |
3.58 |
| 14 |
He, Andrew (10)
|
NIVICT |
51.94 |
53.36 |
1.42 |
| 15 |
Coon, Parker R (10)
|
NIGCOM |
1:00.70 |
1:02.97 |
2.27 |
|
Melendez, Ezekiel I (10)
|
NIRRST |
53.76 |
NS |
- |