| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Judd, Cody (11)
|
NIGRAY |
40.28 |
37.95 |
-2.33 |
| 2 |
Markman, Leopold (12)
|
NIGRAY |
38.33 |
39.40 |
1.07 |
| 3 |
Singer, Jaden (12)
|
NIGRAY |
41.41 |
39.68 |
-1.73 |
| 4 |
Cunningham, Rhys (11)
|
NIGRAY |
42.07 |
39.85 |
-2.22 |
| 5 |
Harrington, William J (11)
|
NIGRAY |
41.54 |
40.44 |
-1.10 |
| 6 |
Spencer, Deven T (12)
|
NIGRAY |
47.04 |
41.14 |
-5.90 |
| 7 |
Wang, Ziao Wang O (11)
|
NIGRAY |
48.58 |
42.02 |
-6.56 |
| 8 |
Kocan, Joshua (12)
|
ZZHRNY |
|
44.33 |
- |
| 9 |
Otero-Vazquez, Santiago (12)
|
NIGRAY |
49.78 |
46.22 |
-3.56 |
| 10 |
Williams, Oliver S (11)
|
NIGRAY |
55.24 |
51.80 |
-3.44 |
| 11 |
Zhang, Alexander Y (11)
|
NIGRAY |
56.49 |
53.45 |
-3.04 |