| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kinnard, Jillian R (12)
|
MICW |
2:36.67 |
2:33.66 |
-3.01 |
| 2 |
Cho, Annika S (12)
|
MIDROP |
2:35.61 |
2:40.11 |
4.50 |
| 3 |
Eng, Mischa C (11)
|
MIGPG |
2:37.60 |
2:41.88 |
4.28 |
| 4 |
Kamara, Bridgette M (12)
|
MICW |
2:52.51 |
2:42.45 |
-10.06 |
| 5 |
Ruggirello, Isabella G (12)
|
MIBBA |
2:46.46 |
2:43.56 |
-2.90 |
| 6 |
Hafner, Kathleen C (11)
|
MIPA |
2:50.75 |
2:47.86 |
-2.89 |
| 7 |
Cusack, Bryn E (11)
|
MIMCA |
2:50.67 |
2:48.02 |
-2.65 |
| 8 |
Jurkiewicz, Lila E (11)
|
MICW |
2:59.25 |
2:50.02 |
-9.23 |
| 9 |
Liggins, Sierra M (12)
|
MIGLT |
2:55.18 |
2:50.26 |
-4.92 |
| 10 |
Vetor, Reese N (11)
|
MIUSSC |
2:57.71 |
2:50.36 |
-7.35 |
| 11 |
Threlkeld, Ella G (11)
|
MIUSSC |
2:43.03 |
2:50.44 |
7.41 |
| 12 |
Azoury, Naya I (12)
|
MIPA |
2:53.96 |
2:50.85 |
-3.11 |
| 13 |
Smith, Lauren M (12)
|
MINS |
2:52.00 |
2:52.93 |
0.93 |
| 14 |
Jurmu, Courtney R (12)
|
MIROCK |
3:05.80 |
2:53.12 |
-12.68 |
| 15 |
Gano, Abigail H (12)
|
OHFMST |
3:01.87 |
2:56.13 |
-5.74 |
| 16 |
Henderson, Emily V (11)
|
MIBBA |
2:58.33 |
2:56.53 |
-1.80 |
| 17 |
Fraeyman, Tia S (12)
|
MIGLT |
2:54.17 |
2:56.76 |
2.59 |
| 18 |
Vrana, Gabriella C (11)
|
MIUSSC |
2:59.87 |
2:57.62 |
-2.25 |
| 19 |
Laba, Jordan N (12)
|
MIOLY |
3:00.60 |
2:57.99 |
-2.61 |
| 20 |
Blakeley, Isabella L (12)
|
MIMD |
3:02.09 |
2:59.47 |
-2.62 |
| 21 |
Lebel, Caitlyn E (12)
|
MIUSSC |
2:59.55 |
3:00.55 |
1.00 |
| 22 |
Pham, Jasmine N (12)
|
MIPCC |
3:02.20 |
3:01.09 |
-1.11 |
| 23 |
Quitos, Abrianna K (12)
|
MIOLY |
3:02.94 |
3:02.92 |
-0.02 |
| 24 |
Hard, Cailey B (11)
|
MIPA |
3:01.74 |
3:03.28 |
1.54 |
| 25 |
Alles, Sasha E (12)
|
MIPAC |
3:05.05 |
3:04.35 |
-0.70 |
| 26 |
Dicks, Madison G (11)
|
MIDROP |
3:04.48 |
3:05.71 |
1.23 |
| 27 |
Rutila, Bridget A (12)
|
MIL |
3:07.79 |
3:12.15 |
4.36 |
| 28 |
Thomas, Addison A (11)
|
MILL |
3:11.53 |
3:12.87 |
1.34 |
| 29 |
Charles, Daisa C (11)
|
MIUN |
3:10.22 |
3:15.41 |
5.19 |