| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Mullins, Rachael A (17)
|
MDHAGY |
26.90 |
28.03 |
1.13 |
| 2 |
Mullins, Meghan N (14)
|
MDHAGY |
27.50 |
28.38 |
0.88 |
| 3 |
Romanowski, Leah M (13)
|
MDHFY |
27.94 |
28.51 |
0.57 |
| 4 |
Jorgensen, Emmabelle (13)
|
MDHFY |
28.35 |
28.75 |
0.40 |
| 5 |
Anderson, Trinity A (12)
|
MDHFY |
28.77 |
28.83 |
0.06 |
| 6 |
Dickerson, Marissa A (14)
|
MDHFY |
29.68 |
29.21 |
-0.47 |
| 7 |
Hudson, Ava K (17)
|
MDHFY |
28.08 |
30.16 |
2.08 |
| 8 |
Nieves, Isabella (14)
|
MDHFY |
29.44 |
30.61 |
1.17 |
| 9 |
Reynoso, Sophia (11)
|
MDHFY |
30.87 |
31.70 |
0.83 |
| 10 |
Romanowski, Ashley A (13)
|
MDHFY |
32.68 |
31.75 |
-0.93 |
| 11 |
Ryu, Esther (13)
|
MDHFY |
31.05 |
31.97 |
0.92 |
| 13 |
Kim, Rachel A (11)
|
MDHFY |
34.40 |
32.64 |
-1.76 |
| 15 |
McPhail, Madison R (13)
|
MDHFY |
34.47 |
35.23 |
0.76 |
| 16 |
Trute, Isabella M (13)
|
MDHFY |
34.25 |
35.25 |
1.00 |
| 17 |
Golba, Brooke T (12)
|
MDHFY |
34.05 |
35.45 |
1.40 |
|
Price, Isabella (15)
|
MDHFY |
29.01 |
NS |
- |
|
Prettyman, Bailey (9)
|
MDHFY |
39.88 |
NS |
- |
|
Johnson, Gisselle K (16)
|
MDHAGY |
32.74 |
NS |
- |
|
McClintock, Jennifer R (13)
|
MDHFY |
37.71 |
NS |
- |
|
Wood, Ashley J (15)
|
MDHFY |
26.69 |
NS |
- |