| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Pavlov, Alissa (12)
|
NEEDGE |
28.58 |
29.08 |
0.50 |
| 2 |
Adams, Michelle E (12)
|
ADCPP |
29.37 |
29.53 |
0.16 |
| 3 |
Hopke, Lillian G (11)
|
ADCPP |
32.33 |
29.85 |
-2.48 |
| 4 |
Kim, Catherine (12)
|
ADSCHE |
31.31 |
31.99 |
0.68 |
| 5 |
Attia, Laila M (12)
|
ADSCHE |
34.34 |
33.89 |
-0.45 |
| 6 |
Hynes, Lillian M (11)
|
ADDELM |
35.66 |
34.16 |
-1.50 |
| 7 |
Polomsky, Alexandra E (12)
|
NEEDGE |
35.34 |
34.19 |
-1.15 |
| 8 |
Moynahan, Maeve C (11)
|
ADSCHE |
36.33 |
34.29 |
-2.04 |
| 9 |
MacLeod, Addison M (12)
|
ADUSAM |
34.25 |
34.30 |
0.05 |
| 10 |
Hou, Irena Y (11)
|
ADDELM |
34.18 |
34.35 |
0.17 |
| 11 |
Taylor, Natalie (12)
|
ADSCHE |
33.80 |
35.10 |
1.30 |
| 12 |
Alonzo, Sophia T (11)
|
ADSARY |
33.57 |
35.14 |
1.57 |
| 13 |
Doig, Isla P (11)
|
NEEDGE |
35.76 |
36.43 |
0.67 |
| 14 |
Ventela, Hannah E (12)
|
ADSARY |
39.05 |
40.49 |
1.44 |
| 15 |
Craig, Kylie A (11)
|
ADSCHE |
43.53 |
41.21 |
-2.32 |
| 16 |
Moore, Grace F (11)
|
ADDELM |
39.04 |
41.35 |
2.31 |
| 17 |
Harder, Phoebe C (11)
|
ADUSAM |
39.32 |
42.87 |
3.55 |
| 18 |
Lape, Cecilia E (12)
|
ADALBS |
|
42.98 |
- |
| 19 |
Quackenbush, Leah H (12)
|
ADALBS |
39.95 |
43.04 |
3.09 |
| 20 |
Windelspecht, Juliana G (12)
|
ADSARY |
45.68 |
45.21 |
-0.47 |
| 21 |
Hu, Ruihan C (11)
|
ADCPP |
51.03 |
48.29 |
-2.74 |
| 22 |
Wells, Olivia M (11)
|
ADBFIN |
|
48.46 |
- |
| 23 |
Silver, Charlotte M (11)
|
ADCPP |
|
50.36 |
- |
| 24 |
Holst, Lily E (12)
|
ADSCHE |
|
50.77 |
- |
| 25 |
Hill, Alice E (11)
|
ADSARY |
53.87 |
52.06 |
-1.81 |
| 26 |
Hendershot, Annalise (11)
|
ADSARY |
|
53.91 |
- |
| 27 |
Goodall, Reagan E (11)
|
ADSCHE |
1:06.93 |
54.51 |
-12.42 |
| 28 |
Wandell, Francelia (11)
|
ADSARY |
|
1:13.88 |
- |
|
Scherer, Scarlett F (12)
|
ADSARY |
1:01.31 |
DQ |
- |
|
Zhang, Cathy H (11)
|
ADCPP |
|
DQ |
- |
|
Christiansen, Adeline J (12)
|
ADUSAM |
|
NS |
- |