| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Amedio, Mea K (15)
|
ADSCHE |
25.39 |
25.21 |
-0.18 |
| 2 |
McLaughlin, Brenna C (17)
|
ADSCHE |
25.30 |
25.80 |
0.50 |
| 3 |
Frame, Ally S (16)
|
ADSCHE |
25.50 |
25.86 |
0.36 |
| 4 |
Nelson, Isabel M (16)
|
ADSCHE |
26.03 |
26.32 |
0.29 |
| 5 |
Versaci, Rosemarie A (17)
|
ADSCHE |
26.16 |
26.81 |
0.65 |
| 6 |
O'Donnell, Maura E (14)
|
ADUN |
26.30 |
26.91 |
0.61 |
| 7 |
O'Loughlin, Natalie G (19)
|
ADSCHE |
27.41 |
27.47 |
0.06 |
| 8 |
Ferrie, Jillian E (14)
|
ADSCHE |
27.48 |
27.87 |
0.39 |
| 9 |
Bogdanowicz-Wilson, Emma K (16)
|
ADSCHE |
27.00 |
28.02 |
1.02 |
| 10 |
Jensen, Jordan G (18)
|
ADSCHE |
|
29.89 |
- |