| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Crouthamel, Lauren C (7)
|
MAWWC |
54.86 |
52.54 |
-2.32 |
| 2 |
Doppler, Molly E (7)
|
MAPRSC |
53.45 |
53.44 |
-0.01 |
| 3 |
Nordberg, Kaitlyn G (7)
|
MAWWC |
56.05 |
54.16 |
-1.89 |
| 4 |
Kelsey, Madison R (7)
|
MAECCC |
1:04.12 |
1:02.56 |
-1.56 |
| 5 |
Geberth, Rebecca L (7)
|
MATHY |
|
1:03.81 |
- |
| 6 |
Petrillo, Jenna A (7)
|
MATWAC |
1:04.22 |
1:10.31 |
6.09 |
| 7 |
Gebhart, Addison F (7)
|
MAPRSC |
|
1:18.04 |
- |
|
Johnson, Olivia M (7)
|
MAFSS |
|
DQ |
- |
|
Sandy, Nora K (7)
|
MAGPAC |
|
DQ |
- |
|
Carroll, Teagan R (7)
|
MASTRM |
|
DQ |
- |
|
Shadman, Sydney E (7)
|
MASTRM |
1:36.04 |
DQ |
- |
|
Blizzard, Mackenzie E (7)
|
MAECCC |
58.97 |
NS |
- |
|
Schroeder, Caroline K (7)
|
MAECCC |
1:13.81 |
NS |
- |
|
Tudor, Daniela C (7)
|
MAFSS |
|
NS |
- |
|
Larish, Eva M (7)
|
MAPRSC |
|
NS |
- |