| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Miller, Breanna R (17)
|
NCTWA |
5:26.18 |
5:14.65 |
-11.53 |
| 2 |
Gould, Ashleigh K (15)
|
SCPALM |
6:09.24 |
5:17.40 |
-51.84 |
| 3 |
Kelly, Ariana E (13)
|
NCUN |
5:26.98 |
5:17.49 |
-9.49 |
| 4 |
Gould, Madison N (15)
|
SCPALM |
5:29.50 |
5:20.76 |
-8.74 |
| 5 |
Dunning, Catherine S (16)
|
NCBAD |
5:22.58 |
5:26.94 |
4.36 |
| 6 |
Kimmel, Allison F (13)
|
NCGG |
5:47.84 |
5:32.13 |
-15.71 |
| 7 |
Perez-Hurtado, Marianna (15)
|
NCMSA |
|
5:37.96 |
- |
| 8 |
Leggat, Caitlyn E (17)
|
NCBAD |
5:59.49 |
5:38.75 |
-20.74 |
| 9 |
Primm, Jett D (17)
|
NCTEAM |
5:24.12 |
5:38.97 |
14.85 |
| 10 |
Evans, Lauren K (14)
|
NCRACY |
6:27.70 |
5:40.93 |
-46.77 |
| 11 |
Edwards, Michaela M (19)
|
NCSAC |
5:45.49 |
5:44.20 |
-1.29 |
| 12 |
Goedl, Monica L (13)
|
NCGG |
5:57.82 |
5:47.95 |
-9.87 |
| 13 |
Blamy, Austyn C (13)
|
NCMSA |
|
5:49.34 |
- |
| 14 |
Thomas, Paige D (12)
|
NCGG |
|
5:52.49 |
- |
| 15 |
Zarins, Chloe G (14)
|
NCTEAM |
6:03.19 |
5:56.06 |
-7.13 |
| 16 |
Jarvis, Alyssa B (15)
|
NCWA |
5:57.89 |
5:57.80 |
-0.09 |
| 17 |
McGuire, Grace M (14)
|
NCRACY |
|
6:05.19 |
- |
| 18 |
Boyter, Brenna E (16)
|
NCGG |
5:55.47 |
6:05.70 |
10.23 |
| 19 |
Matheney, Kenna J (13)
|
NCGG |
|
6:12.88 |
- |
| 20 |
Wheeler, Meghan E (14)
|
NCTEAM |
|
6:19.09 |
- |
| 21 |
Yates, Alexis M (16)
|
NCSAC |
6:15.78 |
6:20.98 |
5.20 |
| 22 |
Bloomfield, Olivia J (13)
|
NCGG |
6:02.98 |
6:23.76 |
20.78 |
| 23 |
Hux, Jillian M (13)
|
NCSAC |
|
6:25.02 |
- |
| 24 |
Wainright, Maris O (13)
|
NCRACY |
6:34.43 |
6:37.07 |
2.64 |
| 25 |
Ellis, Egypt A (15)
|
NCQCD |
|
7:05.66 |
- |
|
Dufton, Stella B (16)
|
NCTEAM |
|
NS |
- |
|
Hutchins, Alicia N (16)
|
NCGG |
5:30.49 |
NS |
- |