| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Barnard, Christopher R (14)
|
LARRAC |
24.46 |
24.25 |
-0.21 |
| 2 |
Tuan, Kenneth (14)
|
STDDST |
24.03 |
24.31 |
0.28 |
| 3 |
Nicolosi, Augustus J (14)
|
LARRAC |
24.71 |
24.94 |
0.23 |
| 4 |
Rhoades, Marcus A (14)
|
LARRAC |
26.64 |
25.82 |
-0.82 |
| 5 |
Joseph, Caleb A (14)
|
LARRAC |
26.42 |
26.78 |
0.36 |
| 6 |
Nguyen, Paul (13)
|
USCSY |
27.35 |
27.21 |
-0.14 |
| 7 |
Cuevas, Juan (13)
|
STDDST |
30.06 |
27.59 |
-2.47 |
| 8 |
Peacock, William F (14)
|
LARRAC |
27.59 |
27.69 |
0.10 |
| 9 |
Rodriguez, Sebastian (13)
|
USCSY |
26.71 |
27.97 |
1.26 |
| 10 |
Beasley, Ryan (13)
|
STDDST |
29.08 |
27.98 |
-1.10 |
| 11 |
Philip, Luke (13)
|
PCY |
26.49 |
28.54 |
2.05 |
| 12 |
Cambell, Zander (13)
|
COPY |
|
29.02 |
- |
| 13 |
Hodson, Trenton (14)
|
COPY |
29.64 |
29.10 |
-0.54 |
| 14 |
Randolph, Parker B (13)
|
LARRAC |
29.09 |
29.90 |
0.81 |
| 15 |
Hasenfratz, Michael (13)
|
PCY |
30.10 |
29.91 |
-0.19 |
| 16 |
Birdsong, Jacob D (13)
|
LARRAC |
30.39 |
30.47 |
0.08 |
| 17 |
Almaguer, Caleb (13)
|
STDDST |
35.19 |
31.28 |
-3.91 |
| 18 |
Ortiz, Kevin (13)
|
STDDST |
31.96 |
31.93 |
-0.03 |
| 19 |
Winston, Rickey A (13)
|
LARRAC |
37.13 |
36.86 |
-0.27 |
| 20 |
Sarvis, Andrew B (13)
|
LARRAC |
43.22 |
39.34 |
-3.88 |