| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
DeBaecke, Sydney E (14)
|
MADST |
57.76 |
59.18 |
1.42 |
| 2 |
Elliott, Sophie (14)
|
PVRMSC |
1:00.99 |
1:01.10 |
0.11 |
| 3 |
Cu, Jasmine N (14)
|
PVRMSC |
1:02.42 |
1:02.11 |
-0.31 |
| 4 |
Cornwell, Jamie G (14)
|
PVOCCS |
1:01.17 |
1:02.45 |
1.28 |
| 5 |
Campbell, Maeve R (14)
|
PVRMSC |
1:03.22 |
1:02.84 |
-0.38 |
| 6 |
Luetkenhaus, Katie M (14)
|
PVOCCS |
59.90 |
1:03.25 |
3.35 |
| 7 |
McHugh, Delaney N (14)
|
MADST |
1:01.65 |
1:03.79 |
2.14 |
| 8 |
Peny, Tess S (14)
|
PVOCCS |
1:01.55 |
1:04.56 |
3.01 |
| 9 |
Chierico, Stella G (14)
|
PVOCCS |
1:03.05 |
1:04.92 |
1.87 |
| 10 |
Fitzgerald, Katelyn G (14)
|
MADST |
1:08.08 |
1:05.06 |
-3.02 |
| 11 |
Sayasithsena, Madeline A (14)
|
PVFISH |
1:01.65 |
1:06.52 |
4.87 |
| 12 |
Boughanem, Hanna Y (14)
|
PVFISH |
1:07.76 |
1:08.69 |
0.93 |
|
Blake, Katherine M (14)
|
PVRMSC |
58.61 |
DQ |
- |