| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Mroczka, Landon W (10)
|
LECSI |
42.71 |
40.13 |
-2.58 |
| 2 |
Polyakov, Andrei (10)
|
LESTRS |
44.88 |
41.98 |
-2.90 |
| 3 |
Jones, Kylen J (10)
|
LESTRS |
48.45 |
42.16 |
-6.29 |
| 4 |
Budiman, Benedictus A (10)
|
LECSI |
43.72 |
42.64 |
-1.08 |
| 5 |
Silcox, Luke J (10)
|
LECSI |
47.04 |
43.61 |
-3.43 |
| 6 |
Chhabra, Sahas (10)
|
LESTRS |
47.12 |
44.04 |
-3.08 |
| 7 |
Aklilu, Nolawi E (10)
|
LETCAT |
47.49 |
44.61 |
-2.88 |
| 8 |
Musick, Ronan J (10)
|
LESTRS |
47.14 |
45.22 |
-1.92 |
| 9 |
Monday, Garrett C (10)
|
LETCAT |
46.14 |
46.19 |
0.05 |
| 10 |
Kang, Aaron (10)
|
LEBBSC |
47.33 |
48.73 |
1.40 |
| 11 |
Greenberg, Gideon S (10)
|
LEGLSS |
55.33 |
49.78 |
-5.55 |
| 12 |
Partee Jr., Breyland P (10)
|
LESTRS |
58.77 |
50.85 |
-7.92 |
| 13 |
Warner, Luke B (10)
|
LEBBSC |
56.77 |
52.39 |
-4.38 |
| 14 |
Sawner, Akshat S (10)
|
LESTRS |
55.36 |
52.52 |
-2.84 |
| 15 |
Port, Micah (10)
|
LEBBSC |
57.93 |
52.95 |
-4.98 |
| 16 |
Cheng, Nicholas D (10)
|
LEBBSC |
59.26 |
54.53 |
-4.73 |
| 17 |
Pandit, Ranveer A (10)
|
LESTRS |
1:10.99 |
1:02.75 |
-8.24 |
| 18 |
Cameron, Ian S (10)
|
LESTRS |
1:03.44 |
1:05.13 |
1.69 |
| 19 |
Koneni, Karunya V (10)
|
LESTRS |
1:00.99 |
1:17.77 |
16.78 |
|
Dietz, Calvin J (10)
|
LELESD |
46.53 |
DQ |
- |