| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gaguzis, Peri S (15)
|
AMKW |
1:00.97 |
1:03.75 |
2.78 |
| 2 |
Patterson, Delaney M (16)
|
AMBVSC |
1:07.25 |
1:06.61 |
-0.64 |
| 3 |
Fontanazza, Callysta L (17)
|
AMUNHA |
1:12.29 |
1:09.41 |
-2.88 |
| 4 |
Stewart, Brooke M (17)
|
AMBVSC |
1:06.84 |
1:09.81 |
2.97 |
| 5 |
Pohodich, Alexandra M (15)
|
AMBVSC |
1:07.32 |
1:10.03 |
2.71 |
| 6 |
Fontanazza, Chloe G (15)
|
AMUNHA |
1:11.65 |
1:10.51 |
-1.14 |
| 7 |
Wagner, Halle E (17)
|
AMUN-M |
1:10.62 |
1:10.97 |
0.35 |
| 8 |
Jackovic, Emily A (16)
|
AMSPAC |
1:09.17 |
1:11.19 |
2.02 |
| 9 |
Glass, Rebecca P (15)
|
AMUN-M |
1:11.37 |
1:14.06 |
2.69 |
| 10 |
Healey, Rebecca J (16)
|
AMBWA |
1:11.11 |
1:17.93 |
6.82 |
| 11 |
Morgan, Audra L (16)
|
AMBVSC |
1:19.27 |
1:18.73 |
-0.54 |
| 12 |
Evans, Danika A (17)
|
AMSPAC |
1:18.99 |
1:18.94 |
-0.05 |