| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Anderson, Stella V (10)
|
NTNTN |
29.23 |
29.59 |
0.36 |
| 2 |
Smetana, Shelby T (8)
|
NTNTN |
35.35 |
34.67 |
-0.68 |
| 3 |
Dukes, Kameron C (10)
|
NTNTN |
39.54 |
34.68 |
-4.86 |
| 4 |
Walser, Georgia H (10)
|
NTNTN |
36.65 |
34.70 |
-1.95 |
| 5 |
Rockafellow, Charlie E (9)
|
NTNTN |
36.44 |
35.21 |
-1.23 |
| 6 |
Franco, Aliana M (8)
|
NTNTN |
38.73 |
36.45 |
-2.28 |
| 7 |
DeYoung, Kay I (10)
|
NTNTN |
38.16 |
36.53 |
-1.63 |
| 8 |
Weilert, Chandler R (9)
|
NTNTN |
39.96 |
37.64 |
-2.32 |
| 9 |
McNary, Kameron K (10)
|
NTNTN |
|
38.60 |
- |
| 10 |
Nguyen, Emma H (9)
|
NTNTN |
43.85 |
43.27 |
-0.58 |
| 11 |
Kizer, Claire R (8)
|
NTNTN |
|
45.48 |
- |
| 12 |
Frost, Ava M (9)
|
NTNTN |
|
46.98 |
- |
| 13 |
Peel, Scarlett E (7)
|
NTNTN |
|
47.10 |
- |
| 14 |
Bartz, Annika M (8)
|
NTNTN |
|
50.98 |
- |
| 15 |
Klopfenstein, Katelyn J (8)
|
NTNTN |
|
1:00.65 |
- |
|
Palmer, Elizabeth J (8)
|
NTNTN |
39.64 |
NS |
- |