| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Van Hout, Kennyn A (9)
|
NISDSC |
49.20 |
48.90 |
-0.30 |
| 2 |
Catalano, Emilee K (10)
|
NIGRAT |
55.19 |
50.67 |
-4.52 |
| 3 |
Garigen, Natalie K (9)
|
NIWEBS |
51.85 |
52.44 |
0.59 |
| 4 |
Walsh, Teagan M (10)
|
NIHAWK |
52.09 |
52.80 |
0.71 |
| 5 |
Gilbert, Natalie J (10)
|
NIUN-V |
52.77 |
52.83 |
0.06 |
| 6 |
Reddington, Hope D (10)
|
NIRRST |
|
52.97 |
- |
| 7 |
Brett, Catherine M (9)
|
NIPACK |
53.82 |
54.93 |
1.11 |
| 8 |
Jensen, Lillian M (10)
|
NIGRSC |
|
57.01 |
- |
| 9 |
Conner, Haley M (9)
|
NIGRAT |
54.95 |
57.62 |
2.67 |
| 10 |
Ciminelli, Sarah P (9)
|
NIGRSC |
|
1:02.65 |
- |
| 11 |
Williams, Lauren K (9)
|
NIHAWK |
1:16.94 |
1:15.91 |
-1.03 |
| 12 |
Galley, Morgan C (10)
|
NIRRST |
1:20.18 |
1:26.01 |
5.83 |
|
Ayers, Brianna S (9)
|
NIGRSC |
|
DQ |
- |
|
Thaney, Brigid G (10)
|
NIGRSC |
1:08.31 |
DQ |
- |
|
McFadden Olivier, Camryn (9)
|
NIPACK |
|
DQ |
- |