| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Dillon, Avery C (12)
|
GUKATY |
2:49.82 |
2:32.42 |
-17.40 |
| 2 |
Quiroz, Ami A (12)
|
GUKATY |
2:37.44 |
2:32.71 |
-4.73 |
| 3 |
Yang, Maggie J (11)
|
GUKATY |
2:40.63 |
2:36.64 |
-3.99 |
| 4 |
Zepeda-Otalora, Alexandra I (12)
|
GUKATY |
2:42.94 |
2:36.96 |
-5.98 |
| 5 |
Curran, Audrey E (12)
|
GUVSST |
2:51.64 |
2:42.18 |
-9.46 |
| 6 |
Feng, Evelyn (11)
|
GUKATY |
2:55.68 |
2:43.04 |
-12.64 |
| 7 |
Miller, Brooke C (12)
|
GUVSST |
2:53.87 |
2:43.42 |
-10.45 |
| 8 |
Strickland, Emma K (12)
|
GUKATY |
2:45.73 |
2:46.60 |
0.87 |
| 9 |
Wernet, Brooke A (11)
|
GUKATY |
3:02.18 |
2:52.35 |
-9.83 |
| 10 |
Beery, Daniela N (12)
|
GUPACK |
3:06.59 |
2:57.42 |
-9.17 |
| 11 |
Myers, Molly E (12)
|
GUPACK |
3:14.20 |
3:01.63 |
-12.57 |
| 12 |
Shekar, Diya (12)
|
GUPACK |
3:06.70 |
3:01.79 |
-4.91 |
| 13 |
Richardson, Evelyn J (12)
|
GUPACK |
3:14.36 |
3:02.56 |
-11.80 |
| 14 |
Gray, Hailey E (12)
|
GUPACK |
3:12.81 |
3:04.44 |
-8.37 |
| 15 |
Qi, Vanessa S (12)
|
GUKATY |
|
3:17.40 |
- |
| 16 |
Molinaro, Liliana E (12)
|
GUPACK |
|
3:42.75 |
- |
| 17 |
Patterson, Alexis L (12)
|
GUPACK |
4:11.01 |
4:08.24 |
-2.77 |
|
Fedorink, Sara A (11)
|
GUPACK |
|
DQ |
- |