| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Stanford, Sydney R (17)
|
GUUN-S |
56.71 |
59.18 |
2.47 |
| 2 |
Jaco, Reagan L (15)
|
GUSSAN |
56.00 |
1:00.17 |
4.17 |
| 3 |
Beardmore, Ashley K (15)
|
GUUN-S |
1:01.25 |
1:05.06 |
3.81 |
| 4 |
Holder, Aislinn G (15)
|
GUUN-S |
1:03.86 |
1:05.20 |
1.34 |
| 5 |
Moriarty, Kiley M (17)
|
GUSSAN |
1:04.71 |
1:07.78 |
3.07 |
| 6 |
Kollatschny, Hannah E (15)
|
GUSSAN |
1:06.61 |
1:10.27 |
3.66 |
| 7 |
Cordes, Lauren E (16)
|
GUSSAN |
1:10.00 |
1:10.89 |
0.89 |
| 8 |
Kohl, Abby L (16)
|
GUUN-S |
1:10.47 |
1:14.33 |
3.86 |
| 9 |
Saria, Noelle D (18)
|
GUTYES |
|
1:14.48 |
- |
| 10 |
Ford, Dawn C (15)
|
GUSST |
|
1:26.83 |
- |
|
Byrd, Audrey A (16)
|
GUSST |
|
NS |
- |
|
Caldwell, Isabelle N (15)
|
GUSSAN |
1:05.30 |
NS |
- |
|
Doyle, Jenna L (17)
|
GUTYES |
1:09.10 |
NS |
- |
|
Longbottom, Charlotte V (16)
|
GUUN-S |
1:01.36 |
NS |
- |