| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Johnson, Madelyn J (12)
|
OZHHST |
36.22 |
38.41 |
2.19 |
| 2 |
Franklin, Layla R (11)
|
ILSPY |
41.05 |
41.84 |
0.79 |
| 3 |
Turk, Erin (11)
|
ILSPY |
41.69 |
42.95 |
1.26 |
| 4 |
O'Brien, Miriam E (11)
|
ILSPY |
43.26 |
43.32 |
0.06 |
| 5 |
Foster, Gwenyth M (11)
|
ILSPY |
47.00 |
45.52 |
-1.48 |
| 6 |
Sreedhar, Arya R (12)
|
ILSPY |
53.18 |
45.92 |
-7.26 |
| 7 |
Leming, Annaliese E (12)
|
ILSPY |
45.66 |
46.11 |
0.45 |
| 8 |
Maender, Ashleigh C (11)
|
ILSPY |
52.05 |
46.14 |
-5.91 |
| 9 |
Poppenhouse, Hannah R (11)
|
ILSPY |
46.50 |
47.22 |
0.72 |
| 10 |
Schaddel, Laurel G (11)
|
ILSPY |
45.58 |
47.76 |
2.18 |
| 11 |
Dey, Maja (12)
|
ILSPY |
45.43 |
47.96 |
2.53 |
| 12 |
Lengacher, Amelia K (12)
|
ILSPY |
47.72 |
48.29 |
0.57 |
| 13 |
Lemon, Alana G (11)
|
OZHHST |
47.73 |
48.38 |
0.65 |
| 14 |
Sarra, Jordin M (12)
|
ILSPY |
44.68 |
48.91 |
4.23 |
| 15 |
Lowney, Andie N (12)
|
OZHHST |
50.00 |
48.97 |
-1.03 |
| 16 |
Matthews, Ella G (12)
|
ILSPY |
|
49.78 |
- |
| 18 |
Deen, Anna M (11)
|
ILSPY |
46.91 |
50.36 |
3.45 |
| 19 |
Pillai, Graceanne B (11)
|
ILSPY |
49.20 |
51.51 |
2.31 |
| 20 |
DeSalle, Ava C (11)
|
ILSPY |
58.29 |
53.00 |
-5.29 |
| 21 |
Metzger, Addison A (11)
|
ILSPY |
52.35 |
53.22 |
0.87 |
| 23 |
Collins, Claire (11)
|
ILSPY |
|
1:09.03 |
- |
|
Madonia, Sophia (11)
|
ILSPY |
50.68 |
DQ |
- |
|
Lancaster, Addison M (11)
|
ILSPY |
58.43 |
DQ |
- |