| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Mullen, Violet E (12)
|
PVNCAP |
36.10 |
35.66 |
-0.44 |
| 2 |
Ohlinger, Danielle S (12)
|
MDCAA |
37.25 |
36.32 |
-0.93 |
| 3 |
Vayra, Emilia A (12)
|
PVNCAP |
37.84 |
37.68 |
-0.16 |
| 4 |
Fong, Emma A (11)
|
PVNCAP |
41.41 |
38.30 |
-3.11 |
| 5 |
Marm, Reagan E (12)
|
PVNCAP |
39.32 |
38.33 |
-0.99 |
| 6 |
Ketler, Ellie R (12)
|
MDCAA |
38.53 |
38.37 |
-0.16 |
| 7 |
Mays, Grace E (11)
|
PVNCAP |
39.57 |
39.06 |
-0.51 |
| 8 |
Cosentino, Elizabeth D (12)
|
MDCAA |
40.48 |
39.15 |
-1.33 |
| 9 |
Hu, Sophia G (12)
|
MDCAA |
40.33 |
39.55 |
-0.78 |
| 10 |
Moran, Neve A (12)
|
MDCAA |
41.10 |
41.06 |
-0.04 |
| 11 |
Vayra, Eva T (11)
|
PVNCAP |
43.89 |
42.20 |
-1.69 |
| 12 |
Hill, Reagan E (11)
|
PVBWST |
38.48 |
42.74 |
4.26 |
| 13 |
Salgado, Eva V (12)
|
PVNCAP |
|
42.99 |
- |
| 14 |
Rufe, McCullough A (11)
|
PVPM |
|
43.03 |
- |
| 15 |
Conway, Matilda J (12)
|
PVNCAP |
|
47.56 |
- |
| 16 |
Glenn, Giuliana E (12)
|
PVNCAP |
|
47.72 |
- |
| 17 |
Maisano, Evelyn R (12)
|
PVPM |
|
48.63 |
- |
| 18 |
Vuyyuru, Chaitra (11)
|
PVDRAG |
|
51.49 |
- |
| 19 |
Vissa, Anika (12)
|
PVPM |
|
53.47 |
- |
|
Griffin, Harper E (11)
|
PVNCAP |
|
NS |
- |
|
Monterrey, Daniella J (12)
|
PVFAST |
|
NS |
- |