| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Marm, Reagan E (12)
|
PVNCAP |
33.36 |
34.39 |
1.03 |
| 2 |
Roth, Victoria M (12)
|
PVNCAP |
38.54 |
34.58 |
-3.96 |
| 3 |
Luna, Olivia C (11)
|
PVNCAP |
41.83 |
34.59 |
-7.24 |
| 4 |
Mays, Grace E (11)
|
PVNCAP |
37.06 |
35.10 |
-1.96 |
| 5 |
Diehl, Hadley R (12)
|
PVNCAP |
37.97 |
35.34 |
-2.63 |
| 6 |
Vayra, Emilia A (12)
|
PVNCAP |
38.24 |
35.57 |
-2.67 |
| 7 |
Salgado, Eva V (12)
|
PVNCAP |
37.91 |
35.95 |
-1.96 |
| 8 |
Mauro, Josephine M (12)
|
MDCAA |
37.08 |
36.21 |
-0.87 |
| 9 |
Hu, Sophia G (12)
|
MDCAA |
37.98 |
37.72 |
-0.26 |
| 10 |
Hebert, Eliana A (12)
|
PVFAST |
36.50 |
37.74 |
1.24 |
| 11 |
Fong, Emma A (11)
|
PVNCAP |
39.99 |
37.86 |
-2.13 |
| 12 |
Chung, Julianne S (12)
|
MDCAA |
37.55 |
38.59 |
1.04 |
| 13 |
Conway, Matilda J (12)
|
PVNCAP |
|
42.95 |
- |
| 14 |
Lutz, Catalina Y (12)
|
PVNCAP |
|
43.47 |
- |
| 15 |
Vayra, Eva T (11)
|
PVNCAP |
43.76 |
43.66 |
-0.10 |
| 16 |
Larese, Alexandra M (12)
|
PVBWST |
39.21 |
44.83 |
5.62 |
| 17 |
Bloom, Madison M (11)
|
PVBWST |
51.85 |
45.36 |
-6.49 |
| 18 |
Hill, Reagan E (11)
|
PVBWST |
37.67 |
46.75 |
9.08 |
| 19 |
Glenn, Giuliana E (12)
|
PVNCAP |
|
50.42 |
- |
| 20 |
Vissa, Anika (12)
|
PVPM |
|
1:24.94 |
- |
|
Griffin, Harper E (11)
|
PVNCAP |
|
NS |
- |
|
Monterrey, Daniella J (12)
|
PVFAST |
|
NS |
- |