| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Singh, Anshika (8)
|
ORLHST |
16.00 |
15.76 |
-0.24 |
| 2 |
Chamberland, Brienne G (8)
|
ORSSS |
16.35 |
15.96 |
-0.39 |
| 3 |
Liu, Beatrice E (8)
|
ORMAC |
15.96 |
16.07 |
0.11 |
| 4 |
Borus, Alexandra M (8)
|
ORMAC |
15.89 |
16.17 |
0.28 |
| 5 |
Pop, Natalie B (8)
|
ORCS |
16.32 |
16.54 |
0.22 |
| 6 |
Schmidt, Presley K (8)
|
OROCST |
16.93 |
16.55 |
-0.38 |
| 7 |
Morita, Gabriela R (7)
|
ORLHST |
17.33 |
16.63 |
-0.70 |
| 8 |
Lindsly, Nola K (8)
|
ORLOSC |
16.78 |
16.81 |
0.03 |
| 9 |
Winter, Ayana F (8)
|
ORPAC |
17.03 |
16.86 |
-0.17 |
| 10 |
Sullivan, Kailee R (8)
|
ORHEAT |
17.59 |
17.00 |
-0.59 |
| 11 |
Montang, Madelyn M (8)
|
ORTTSC |
16.85 |
17.10 |
0.25 |
| 12 |
Bachmeier, Olivia L (8)
|
ORLOSC |
16.69 |
17.11 |
0.42 |
| 13 |
Middlestadt, Peyton H (8)
|
ORCA |
17.00 |
17.22 |
0.22 |
| 14 |
Miller, Madeleine G (7)
|
ORVSST |
17.56 |
17.39 |
-0.17 |
| 14 |
Gallagher, Lucy A (8)
|
ORMHA |
17.40 |
17.39 |
-0.01 |
| 16 |
Penny, Clara J (8)
|
ORPAC |
17.96 |
17.51 |
-0.45 |
| 17 |
Apoorvi, Preksha (8)
|
ORCS |
17.61 |
17.72 |
0.11 |
| 18 |
Lovrien, Kaylee A (8)
|
ORRACE |
19.85 |
17.73 |
-2.12 |
| 19 |
Garyfallou, Ekaterini A (7)
|
ORMAC |
17.72 |
17.76 |
0.04 |
| 20 |
Yalamarthi, Hansitha * (8)
|
ORLOSC |
17.66 |
17.77 |
0.11 |
| 21 |
Miller, Isla M (8)
|
ORLHST |
17.47 |
18.37 |
0.90 |
| 22 |
Blackhurst, Callie A (8)
|
ORTHSC |
18.00 |
19.16 |
1.16 |
| 23 |
Montgomery, Alexa J (8)
|
ORCAT |
17.50 |
19.98 |
2.48 |