| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Chamberland, Brienne G (8)
|
ORSSS |
43.63 |
43.46 |
-0.17 |
| 2 |
Liu, Beatrice E (8)
|
ORMAC |
44.70 |
44.61 |
-0.09 |
| 3 |
Kalinda-Melcher, Lydia-Mondé (8)
|
ORPAC |
47.39 |
45.40 |
-1.99 |
| 4 |
Huang, Annika E (8)
|
ORBEND |
52.26 |
47.47 |
-4.79 |
| 5 |
Koch, Samantha N (7)
|
ORLOSC |
48.94 |
48.99 |
0.05 |
| 6 |
Pederson, Betsey A (8)
|
ORTOR |
52.26 |
50.02 |
-2.24 |
| 7 |
Jackson, Sydney L (8)
|
ORLHST |
49.60 |
50.25 |
0.65 |
| 8 |
Demaray, Karsyn N (8)
|
ORCRST |
52.29 |
50.31 |
-1.98 |
| 9 |
Hutton, Peyton M (8)
|
ORTTSC |
52.02 |
51.28 |
-0.74 |
| 10 |
Sandhu, Aryana A (8)
|
ORTHSC |
51.58 |
51.43 |
-0.15 |
| 11 |
Montang, Madelyn M (8)
|
ORTTSC |
51.20 |
51.50 |
0.30 |
| 12 |
Yalamarthi, Hansitha * (8)
|
ORLOSC |
52.52 |
51.65 |
-0.87 |
| 13 |
Apoorvi, Preksha (8)
|
ORCS |
52.91 |
51.75 |
-1.16 |
| 14 |
Montgomery, Emma I (8)
|
ORHEAT |
53.41 |
52.85 |
-0.56 |
| 15 |
Morita, Gabriela R (7)
|
ORLHST |
50.82 |
53.15 |
2.33 |
|
Noryang, Tenzin (8)
|
ORHEAT |
50.63 |
DQ |
- |
|
Pop, Natalie B (8)
|
ORCS |
51.74 |
DQ |
- |