| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Harper, Amelia D (13)
|
NIHHSC |
27.73 |
27.90 |
0.17 |
| 2 |
Moppert, Tia M (14)
|
NIGWST |
28.80 |
28.22 |
-0.58 |
| 3 |
Fortna, Julia M (14)
|
NIHHSC |
29.14 |
29.39 |
0.25 |
| 4 |
Fortna, Jane E (14)
|
NIHHSC |
30.01 |
30.26 |
0.25 |
| 5 |
Delaney, Carley A (13)
|
NISWAT |
30.20 |
30.53 |
0.33 |
| 6 |
Rasha, Jayla J (14)
|
NISAAC |
33.75 |
30.78 |
-2.97 |
| 7 |
Aldrich, Gabrielle E (14)
|
NIHHSC |
30.48 |
30.94 |
0.46 |
| 8 |
Yarbour, Jem O (13)
|
NISAAC |
35.31 |
32.37 |
-2.94 |
| 9 |
Yetter, Samantha M (14)
|
NISWAT |
32.95 |
32.97 |
0.02 |
| 10 |
Dinse, Lilia P (14)
|
NISWAT |
33.91 |
33.24 |
-0.67 |
| 11 |
Redeye, Elsie I (13)
|
NISAAC |
34.92 |
33.94 |
-0.98 |
| 12 |
Sternisha, Madison N (13)
|
NIGWST |
34.89 |
35.13 |
0.24 |
|
Stang, Hayley M (13)
|
NIGWST |
33.24 |
NS |
- |
|
Gould, Lillee G (14)
|
NIHHSC |
27.99 |
NS |
- |