| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Balzano, Gioia R (10)
|
FGEA |
5:35.52 |
5:31.45 |
-4.07 |
| 2 |
Gregory, Elizabeth F (10)
|
VAWAC |
5:42.59 |
5:35.75 |
-6.84 |
| 3 |
Gotham, Ella M (10)
|
MDHFY |
5:46.23 |
5:39.55 |
-6.68 |
| 4 |
Callis, Emerson P (10)
|
VAQSTS |
5:50.89 |
5:50.22 |
-0.67 |
| 5 |
Block, Alyse (10)
|
WIOCSC |
5:49.38 |
5:50.49 |
1.11 |
| 6 |
Dietschweiler, Kiersten K (10)
|
WIOSHY |
5:51.95 |
5:51.82 |
-0.13 |
| 7 |
McCoyd, Alaina E (10)
|
MDFSC |
5:59.87 |
5:53.37 |
-6.50 |
| 8 |
Markoja, Addison (10)
|
CTSEAL |
5:57.45 |
6:00.12 |
2.67 |
| 9 |
Gruber, Zoe A (10)
|
WIUN-0 |
6:05.18 |
6:00.72 |
-4.46 |
| 10 |
Haas, Abigail D (10)
|
AMKW |
6:09.75 |
6:08.85 |
-0.90 |
| 11 |
Hansen, Karrington L (10)
|
FLSPA |
6:07.55 |
6:14.92 |
7.37 |
| 12 |
Sorensen, Audrey R (10)
|
MDNBAC |
6:10.53 |
6:20.57 |
10.04 |
| 13 |
Steves, Madeleine A (10)
|
PVFISH |
6:07.81 |
6:27.70 |
19.89 |
|
Osborn, Karissa N (10)
|
OZEDWY |
6:35.49 |
6:25.19 |
-10.30 |