| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Gotham, Ella M (10)
|
MDHFY |
2:29.94 |
2:25.95 |
-3.99 |
| 2 |
Gregory, Elizabeth F (10)
|
VAWAC |
2:27.12 |
2:27.14 |
0.02 |
| 3 |
Callis, Emerson P (10)
|
VAQSTS |
2:27.22 |
2:29.60 |
2.38 |
| 4 |
Balzano, Gioia R (10)
|
FGEA |
2:27.40 |
2:29.64 |
2.24 |
| 5 |
Bendis, Carley J (10)
|
MDNBAC |
2:33.07 |
2:32.39 |
-0.68 |
| 6 |
Block, Alyse (10)
|
WIOCSC |
2:52.48 |
2:33.81 |
-18.67 |
| 7 |
Dietschweiler, Kiersten K (10)
|
WIOSHY |
2:30.79 |
2:34.00 |
3.21 |
| 8 |
McCoyd, Alaina E (10)
|
MDFSC |
2:33.42 |
2:34.36 |
0.94 |
| 9 |
Schrecongost, Kasey R (10)
|
AMKW |
2:33.24 |
2:35.26 |
2.02 |
| 10 |
Gruber, Zoe A (10)
|
WIUN-0 |
2:35.07 |
2:35.30 |
0.23 |
| 11 |
Furlow, Chrissy J (10)
|
MDNBAC |
2:41.38 |
2:35.77 |
-5.61 |
| 12 |
Oliver, Olivia A (10)
|
PVOCCS |
2:36.61 |
2:36.17 |
-0.44 |
| 13 |
Menely, Payton (10)
|
ILAA |
2:42.25 |
2:36.81 |
-5.44 |
| 14 |
Sorensen, Audrey R (10)
|
MDNBAC |
2:36.39 |
2:37.62 |
1.23 |
| 15 |
Fulton, Zoee H (10)
|
FLH4S |
2:40.61 |
2:37.89 |
-2.72 |
| 16 |
Lehman, Alyce E (10)
|
MAJW |
2:36.35 |
2:39.01 |
2.66 |
| 17 |
Steves, Madeleine A (10)
|
PVFISH |
2:38.63 |
2:41.13 |
2.50 |
| 18 |
Sanders, Janae D (10)
|
FLPYP |
2:36.04 |
2:41.38 |
5.34 |
| 19 |
Weingarten, Elise A (10)
|
MNRSC |
2:40.16 |
2:43.17 |
3.01 |
| 20 |
Hansen, Karrington L (10)
|
FLSPA |
2:50.93 |
2:43.59 |
-7.34 |
| 21 |
Calone, Emilia R (10)
|
MRMWDA |
2:39.65 |
2:43.77 |
4.12 |