| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Kohl, Rayburn (12)
|
ILMYST |
27.46 |
27.85 |
0.39 |
| 2 |
Bergren, Anders M (12)
|
ILHEAT |
27.46 |
27.93 |
0.47 |
| 3 |
Lapys, Augustas (12)
|
ILLATT |
29.05 |
29.16 |
0.11 |
| 4 |
Zhu, Ethan (11)
|
ILLATT |
29.40 |
29.62 |
0.22 |
| 5 |
Rupniewski, Simon P (12)
|
ILSAGE |
30.21 |
29.74 |
-0.47 |
| 6 |
Sullens, Lucas J (12)
|
ILSPY |
30.04 |
29.91 |
-0.13 |
| 7 |
Hadjistamoulou, Anestis (11)
|
ILLATT |
32.02 |
30.40 |
-1.62 |
| 8 |
Marks, Andrew S (12)
|
ILSAGE |
30.70 |
30.64 |
-0.06 |
| 9 |
Mizeur, Rowan (11)
|
ILSPY |
30.48 |
30.71 |
0.23 |
| 10 |
Gao, Robert * (12)
|
ILHEAT |
31.24 |
30.79 |
-0.45 |
| 11 |
Lowery, Brian D (11)
|
ILILVY |
32.80 |
30.82 |
-1.98 |
| 12 |
Shewfelt, Finch p (11)
|
ILMYST |
31.79 |
30.90 |
-0.89 |
| 13 |
Tang, Bruce R (12)
|
ILHEAT |
31.23 |
31.19 |
-0.04 |
| 14 |
Dolan, Matthew B (12)
|
ILRAYS |
32.38 |
31.72 |
-0.66 |
| 15 |
Denny, Roe (12)
|
ZZCANY |
32.01 |
31.75 |
-0.26 |
| 16 |
Salas, Ernesto (12)
|
ILPAV |
33.05 |
31.77 |
-1.28 |
| 17 |
Peng, Isaac (12)
|
ILMYST |
31.67 |
31.88 |
0.21 |
| 18 |
Dante, Jacob M (12)
|
ILJETS |
33.08 |
32.04 |
-1.04 |
| 19 |
Rayford, Warren K (12)
|
ILDOCS |
31.84 |
32.08 |
0.24 |
| 20 |
Fedorenko, Maximus A (12)
|
ILLATT |
32.72 |
32.14 |
-0.58 |
| 21 |
Faulkner, Alexander (12)
|
ILDANY |
32.24 |
32.17 |
-0.07 |
| 22 |
Kearns, Owen B (12)
|
ILHEAT |
31.68 |
32.33 |
0.65 |
| 23 |
Hutchinson, Noah P (12)
|
ILBNY |
32.05 |
32.45 |
0.40 |
| 24 |
Daman, Cameron D (11)
|
ILSAGE |
33.04 |
32.70 |
-0.34 |
| 25 |
Rivera, Alejandro A (12)
|
ILSPY |
32.52 |
32.79 |
0.27 |
| 26 |
Hovey, William A (12)
|
ILSPY |
31.10 |
32.81 |
1.71 |
| 27 |
Praczkowski, Victor (11)
|
ILSAGE |
32.55 |
32.84 |
0.29 |
| 28 |
Medlin, Charles (11)
|
ILDANY |
32.49 |
33.01 |
0.52 |
| 29 |
Olson, Carson (12)
|
KNCY |
32.82 |
33.05 |
0.23 |
| 30 |
De La Cruz, Samuel S (11)
|
ILDCST |
33.06 |
33.19 |
0.13 |
| 31 |
Skrzypek, Alexander E (11)
|
ILDCST |
32.94 |
33.22 |
0.28 |
|
Morton, Robert L (12)
|
ILSAGE |
31.77 |
NS |
- |