| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Veerapaneni, Jaya N (14)
|
ILLWSA |
2:45.60 |
2:41.82 |
-3.78 |
| 2 |
Kubota, Nonoka (13)
|
ILLWSA |
2:55.26 |
2:45.75 |
-9.51 |
| 3 |
Marcotte, Isabelle C (14)
|
INLCB |
2:54.76 |
2:47.67 |
-7.09 |
| 4 |
Abdulla, Amana L (14)
|
INLCB |
2:51.79 |
2:51.07 |
-0.72 |
| 5 |
Meldeau, Sarah A (14)
|
ILLWSA |
3:11.21 |
2:51.33 |
-19.88 |
| 6 |
Pacheco, Brenna J (14)
|
INLCB |
2:55.60 |
2:53.63 |
-1.97 |
| 7 |
Zenow, Anna G (13)
|
ILLIFE |
3:05.34 |
2:55.27 |
-10.07 |
| 8 |
Trautvetter, Kylie M (14)
|
ILLWSA |
2:50.31 |
2:55.89 |
5.58 |
| 9 |
Murphy, Claire E (13)
|
ILLWSA |
3:15.69 |
3:01.10 |
-14.59 |
| 10 |
Johnson, Kaylee E (14)
|
ILNAC |
3:19.11 |
3:03.88 |
-15.23 |
| 11 |
Barker, Callie M (13)
|
ILLWSA |
3:23.03 |
3:04.29 |
-18.74 |
| 12 |
Vollmer, Emily M (13)
|
ILFVFY |
3:20.15 |
3:04.60 |
-15.55 |
| 13 |
Olson, Molly N (14)
|
ILLWSA |
3:13.40 |
3:06.96 |
-6.44 |
| 14 |
Earll, Mia J (13)
|
INMSC |
|
3:09.98 |
- |
| 15 |
Wesner, Hannah (13)
|
INMSC |
3:32.82 |
3:11.59 |
-21.23 |
| 16 |
Dumaresq, Lauren J (14)
|
INMSC |
|
3:14.45 |
- |
| 17 |
Maces, Melecia L (13)
|
ILFVFY |
4:06.36 |
3:15.58 |
-50.78 |
| 18 |
Lehman, Victoria A (13)
|
ILLWSA |
3:06.95 |
3:15.59 |
8.64 |
| 19 |
Abdulla, Aalia (13)
|
INLCB |
3:43.65 |
3:15.93 |
-27.72 |
| 20 |
Adducci, Siena D (13)
|
ILLWSA |
|
3:17.05 |
- |
| 21 |
Dykshorn, Dana O (14)
|
ILLWSA |
|
3:20.64 |
- |
| 22 |
Powell, Olivia M (14)
|
ILLWSA |
4:39.57 |
3:21.09 |
-78.48 |
| 23 |
Schultz, Victoria E (13)
|
ILFVFY |
|
3:57.77 |
- |
| 24 |
Glens, Lauren M (13)
|
ILLWSA |
|
4:06.65 |
- |
|
Hagwell, Madilyn M (14)
|
ILLWSA |
|
DQ |
- |
|
Kim, Faustina M (13)
|
INLCB |
3:45.37 |
DQ |
- |
|
Jordan, Bernadette M (13)
|
ILFVFY |
|
DQ |
- |