| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Harkema, Abigail S (15)
|
INLCB |
2:42.80 |
2:52.17 |
9.37 |
| 2 |
Rhodes, Samantha M (18)
|
ILWSO |
2:49.36 |
2:56.14 |
6.78 |
| 3 |
Stasny, Taylor A (15)
|
INLCB |
2:49.26 |
3:01.20 |
11.94 |
| 4 |
Cichon, Isabella J (16)
|
ILLWSA |
2:48.81 |
3:06.09 |
17.28 |
| 5 |
Fernandez, Natasha N (15)
|
ILMMAC |
3:14.01 |
3:08.83 |
-5.18 |
| 6 |
Marchese, Lena K (16)
|
ILCWAC |
3:13.86 |
3:08.89 |
-4.97 |
| 7 |
Johnson, Chloe D (17)
|
ILWCS |
3:08.30 |
3:09.56 |
1.26 |
| 8 |
Chesney, Cora E (16)
|
ILMMAC |
3:18.36 |
3:15.51 |
-2.85 |
| 9 |
Eng, Meghan E (15)
|
ILTIDE |
|
3:16.01 |
- |
| 10 |
Smitendorf, Julia M (16)
|
ILMMAC |
3:23.43 |
3:22.34 |
-1.09 |
| 11 |
Hubster, Ava R (16)
|
INCPSC |
3:18.57 |
3:22.81 |
4.24 |
| 12 |
Hutchison-Simpson, Lilly E (17)
|
INUTSC |
|
3:37.56 |
- |
| 13 |
Ewanic, Sophia J (15)
|
ILLWSA |
3:55.86 |
3:45.26 |
-10.60 |
|
Hatcher, Ashley N (16)
|
ILFVFY |
3:11.28 |
NS |
- |
|
Fiske, Delaney J (15)
|
ILLWSA |
|
NS |
- |