| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Guzman, Hannah E (12)
|
VAPWSC |
32.62 |
31.13 |
-1.49 |
| 2 |
Warner, Ariana M (12)
|
VAPWSC |
36.76 |
33.25 |
-3.51 |
| 3 |
Segura, Mara J (12)
|
VASTAT |
33.42 |
33.68 |
0.26 |
| 4 |
Irgens, Marit N (12)
|
VAWST |
35.00 |
34.13 |
-0.87 |
| 5 |
Sheehan, Jessica R (11)
|
VASTAT |
34.45 |
34.30 |
-0.15 |
| 6 |
Truong, Aline C (11)
|
VAWST |
44.39 |
34.38 |
-10.01 |
| 7 |
Mumley, Reese P (11)
|
VAPWSC |
38.03 |
35.43 |
-2.60 |
| 8 |
Royal, Sydney S (12)
|
VAPWSC |
37.21 |
35.90 |
-1.31 |
| 9 |
Saha, Adritaa R (12)
|
VAPWSC |
37.60 |
36.41 |
-1.19 |
| 10 |
Kim, Shane E (12)
|
VAWST |
52.54 |
37.07 |
-15.47 |
| 11 |
Osorio, Mariana (12)
|
VAPWSC |
38.05 |
37.78 |
-0.27 |
| 12 |
Holland, Samantha C (12)
|
VATSU |
42.07 |
37.84 |
-4.23 |
| 13 |
Bodie, Emma C (10)
|
VAPWSC |
|
40.56 |
- |
| 14 |
Heston, Kate L (12)
|
VATSU |
39.72 |
41.57 |
1.85 |
| 15 |
Turner, Kayden D (12)
|
VAPWSC |
41.99 |
43.18 |
1.19 |
| 16 |
Kitta, Julia M (9)
|
VAPWSC |
49.71 |
46.06 |
-3.65 |
| 17 |
Domingo, Alexa M (10)
|
VAPWSC |
|
48.77 |
- |
| 18 |
Wells, Devyn C (12)
|
VAPWSC |
49.75 |
51.33 |
1.58 |
| 19 |
Avolio, Kara J (11)
|
VAPWSC |
56.15 |
53.13 |
-3.02 |
| 20 |
Bukarau, Adi Lenikali B (11)
|
VAPWSC |
54.70 |
55.26 |
0.56 |
| 21 |
Benz Graham, Shyanna D (12)
|
VAPWSC |
|
55.43 |
- |
| 22 |
Durham, Ariella R (9)
|
VAPWSC |
52.87 |
56.33 |
3.46 |
| 23 |
Price, Amelia G (10)
|
VASTAT |
|
56.99 |
- |
| 24 |
Billings, Grace M (12)
|
VAWST |
49.51 |
1:03.91 |
14.40 |
| 25 |
Flynn, Annabel P (10)
|
VAPWSC |
|
1:04.32 |
- |
| 26 |
Forbes, Edna B (11)
|
VATSU |
|
1:14.51 |
- |
| 27 |
Arbeiter, Emma E (9)
|
VAPWSC |
|
1:14.52 |
- |
|
Hurley, Georgina H (10)
|
VAPWSC |
|
DQ |
- |
|
Berg, Christiana A (10)
|
VAPWSC |
|
NS |
- |
|
Miller, Eden E (10)
|
VASTAT |
|
NS |
- |
|
Roberts-Shaw, Myliah S (12)
|
VAWST |
52.83 |
NS |
- |
|
MacDougall, Lily E (9)
|
VAPWSC |
|
NS |
- |