| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Watschke, Madison (10)
|
MASCAY |
38.92 |
37.39 |
-1.53 |
| 2 |
Chen, Emily Y (9)
|
MASCAY |
39.54 |
39.47 |
-0.07 |
| 3 |
Watschke, Katelyn (10)
|
MASCAY |
39.52 |
41.68 |
2.16 |
| 4 |
Becker, Kyla E (9)
|
JSY |
43.43 |
42.85 |
-0.58 |
| 5 |
Williams, Lilyana (9)
|
MASCAY |
47.83 |
44.75 |
-3.08 |
| 6 |
Lee, Chanmi (10)
|
MASCAY |
|
44.88 |
- |
| 7 |
Delaney, Charlotte (9)
|
MASCAY |
42.98 |
45.10 |
2.12 |
| 8 |
Boomer-Brazier, Maggie (10)
|
MASCAY |
45.88 |
46.32 |
0.44 |
| 9 |
Robbins, Makenna (9)
|
JSY |
56.07 |
48.78 |
-7.29 |
| 10 |
Lose, Jaylee (10)
|
ENBEFY |
51.76 |
53.05 |
1.29 |
| 11 |
Congiu-Hughes, Chiara (9)
|
MASCAY |
50.01 |
53.85 |
3.84 |
| 12 |
Harchak, Ava A (9)
|
ENBEFY |
1:05.34 |
1:09.21 |
3.87 |
| 13 |
Laird, Serenity (10)
|
JSY |
1:34.97 |
1:33.26 |
-1.71 |
|
Belz, Kristina (9)
|
JSY |
1:06.01 |
DQ |
- |