| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wiggins, Jack D (14)
|
MDGBSA |
26.90 |
26.92 |
0.02 |
| 2 |
Kinsey, Kaden J (14)
|
MDASC |
27.57 |
27.96 |
0.39 |
| 3 |
Kelley, Nathaniel T (14)
|
MDSPRC |
29.96 |
28.44 |
-1.52 |
| 4 |
Hughes, Ryan F (14)
|
MDACA |
|
28.88 |
- |
| 5 |
Ademola, Oluwaseun J (14)
|
MDFSC |
29.64 |
29.48 |
-0.16 |
| 6 |
Hayburn, Luke G (14)
|
MDASC |
30.48 |
29.61 |
-0.87 |
| 7 |
Ward, Parker A (14)
|
MDASC |
29.60 |
29.65 |
0.05 |
| 8 |
Marston, Samuel A (14)
|
MDFSC |
29.70 |
29.76 |
0.06 |
| 9 |
Harsanyi, Dominick (13)
|
MDFSC |
30.62 |
30.12 |
-0.50 |
| 10 |
Vance, David A (14)
|
MDFSC |
30.98 |
30.63 |
-0.35 |
| 11 |
Gao, Daniel W (14)
|
PVRY |
31.74 |
31.00 |
-0.74 |
| 12 |
Campbell, Satya C (13)
|
MDLBA |
32.64 |
31.03 |
-1.61 |
| 13 |
Walter, Jackson (14)
|
MDSMDA |
|
31.34 |
- |
| 14 |
Lamprey, Camden A (13)
|
MDSPRC |
31.35 |
31.60 |
0.25 |
| 15 |
Mitchell, Finley R (13)
|
MDLBA |
35.66 |
32.01 |
-3.65 |
| 16 |
Winters, Kyle D (13)
|
MDSPRC |
33.37 |
32.49 |
-0.88 |
| 17 |
Garner, Nathan D (13)
|
MDACA |
|
32.98 |
- |
| 18 |
Kraft, Gabriel M (13)
|
MDSPRC |
34.93 |
33.37 |
-1.56 |
| 19 |
Miller, Bennett P (14)
|
MDFSC |
41.86 |
33.40 |
-8.46 |
| 20 |
Garvey, Henry C (13)
|
MDASC |
|
33.80 |
- |
| 21 |
Ratte, Jonathan E (13)
|
MDASC |
34.05 |
34.01 |
-0.04 |
| 22 |
Wang, Nathan N (13)
|
PVRY |
|
34.29 |
- |
| 23 |
Nash, Gray A (14)
|
MDSPRC |
35.54 |
34.81 |
-0.73 |
| 24 |
Kurup, Pranav (13)
|
MDGBSA |
|
36.44 |
- |
| 25 |
Gwin, Jack T (13)
|
MDACA |
|
39.65 |
- |
| 26 |
Smith, Odin J (14)
|
MDFSC |
48.10 |
40.63 |
-7.47 |
| 27 |
Klimov, Gleb N (13)
|
MDGBSA |
|
42.35 |
- |
| 28 |
Chernyakhovsky, Nikita N (13)
|
MDACA |
|
43.07 |
- |
|
Hertzler, Leeland (13)
|
MDSMDA |
|
NS |
- |
|
Malikov, Michael N (14)
|
MDSPRC |
26.38 |
NS |
- |