| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Richardson, Evelyn J (11)
|
GUPACK |
2:41.55 |
2:40.87 |
-0.68 |
| 2 |
Wood, Hadley K (12)
|
GUAGS |
2:54.37 |
2:44.53 |
-9.84 |
| 3 |
Riddle, Gloria (11)
|
GUAGS |
2:53.52 |
2:48.86 |
-4.66 |
| 4 |
Garcia, Alayda I (12)
|
GUNOCH |
2:49.86 |
2:51.56 |
1.70 |
| 5 |
Garcia, Ava E (12)
|
GUNOCH |
2:52.66 |
2:52.11 |
-0.55 |
| 6 |
Janak, Peyton E (12)
|
GUPLAT |
3:01.77 |
2:52.50 |
-9.27 |
| 7 |
Kolmel-Vieira, Isabella (11)
|
GUDADS |
3:07.18 |
2:53.75 |
-13.43 |
| 8 |
Rubin, Sarah (12)
|
GUKATY |
3:01.51 |
2:57.89 |
-3.62 |
| 9 |
Donkers, Kathelijne F (11)
|
GUDADS |
3:06.55 |
2:57.92 |
-8.63 |
| 10 |
Salazar, Zoe D (11)
|
GUPACK |
2:59.38 |
2:59.01 |
-0.37 |
| 11 |
Harvin, Kacey E (11)
|
GUDADS |
2:58.53 |
2:59.61 |
1.08 |
| 12 |
Shaw, Isabella M (11)
|
GUPLAT |
2:59.03 |
3:00.97 |
1.94 |
| 13 |
Holden, Michelle M (12)
|
GUPFL |
3:19.86 |
3:03.59 |
-16.27 |
| 14 |
Sherrer, Haley R (12)
|
GUPACK |
|
3:18.18 |
- |
| 15 |
Austin, Gabrielle A (12)
|
GUSPA |
3:16.89 |
3:21.66 |
4.77 |
| 16 |
Harren, Caroline N (11)
|
GUDADS |
3:17.32 |
3:25.16 |
7.84 |
| 17 |
Patterson, Alexis L (12)
|
GUPACK |
|
3:32.92 |
- |
| 18 |
Brown, Alyssa (11)
|
GUEPRC |
|
3:44.35 |
- |
|
Canovas, Mirielle W (12)
|
GUPACK |
2:59.82 |
NS |
- |
|
Diamond, Addison K (11)
|
GUKATY |
2:42.84 |
NS |
- |
|
Young, Nicole C (12)
|
GUDADS |
2:46.25 |
NS |
- |
|
Jones, Berit G (12)
|
GUDADS |
|
NS |
- |