| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
McCormick, Riley E (12)
|
FGCSSC |
44.24 |
37.20 |
-7.04 |
| 2 |
Robertson, Emily R (12)
|
FGCSSC |
37.66 |
38.19 |
0.53 |
| 3 |
Leete, Siera A (12)
|
FGSFTL |
40.50 |
38.20 |
-2.30 |
| 4 |
Rilo, Illari (12)
|
FGSUN |
39.72 |
38.89 |
-0.83 |
| 5 |
Wells, Hailey I (12)
|
FGCSSC |
42.02 |
39.42 |
-2.60 |
| 6 |
Troccoli, Isabella M (12)
|
FGSUN |
39.01 |
39.74 |
0.73 |
| 7 |
Renaud, Eva (12)
|
FGSF |
41.70 |
40.22 |
-1.48 |
| 8 |
Levin, Rebeca (12)
|
FGSF |
42.83 |
40.83 |
-2.00 |
| 9 |
Smith, Megan R (12)
|
FGPST |
40.12 |
40.90 |
0.78 |
| 10 |
Landron, Teresa M (12)
|
FGSUN |
44.81 |
41.44 |
-3.37 |
| 11 |
Olson, Elisabeth G (12)
|
FGCSSC |
44.16 |
41.61 |
-2.55 |
| 12 |
Chinogwenya, Nakai S (12)
|
FGSF |
40.89 |
42.10 |
1.21 |
| 13 |
Kaplan, Kate S (12)
|
FGCCPR |
45.32 |
42.38 |
-2.94 |
| 14 |
Ward, Payton A (12)
|
FGCSSC |
43.27 |
42.53 |
-0.74 |
| 15 |
Hockman, Madison P (12)
|
FGCSSC |
45.28 |
44.99 |
-0.29 |
| 16 |
Gonzalez, Breanna (12)
|
FGSUN |
51.23 |
45.24 |
-5.99 |
| 17 |
Palacios, Nicole (12)
|
FGCSSC |
47.90 |
46.51 |
-1.39 |
| 18 |
Banks, Brooklyn (12)
|
FGSF |
46.71 |
46.61 |
-0.10 |
| 19 |
Sapp, Ysabella N (12)
|
FGCSSC |
1:01.71 |
46.88 |
-14.83 |
| 20 |
Kuncas, Holly L (12)
|
FGSFTL |
|
47.12 |
- |
| 21 |
Walker, Syven N (12)
|
FGSUN |
|
47.18 |
- |
| 22 |
Pottigari, Saanvi R (12)
|
FGSOFL |
|
48.83 |
- |
| 23 |
Grisby, Kennedy C (12)
|
FGCSSC |
|
48.96 |
- |
| 24 |
Voit, Catherine (12)
|
FGCSSC |
57.73 |
1:00.62 |
2.89 |
| 25 |
Swack, Meaghan J (12)
|
FGCSSC |
|
1:03.04 |
- |
| 26 |
Suresh Kumar, Sai Neha (12)
|
FGSUN |
|
1:06.30 |
- |
|
Solis, Samantha (12)
|
FGSOFL |
48.27 |
NS |
- |
|
Pierre-Antoine, Eva V (12)
|
FGCCPR |
41.35 |
NS |
- |