| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Palacios, Camila (10)
|
ILDCST |
35.14 |
36.43 |
1.29 |
| 2 |
Drent, Payton M (10)
|
ILFVFY |
45.92 |
41.57 |
-4.35 |
| 3 |
Fredrickson, Jessica G (9)
|
ILDCST |
45.87 |
44.23 |
-1.64 |
| 4 |
Short, Emma J (10)
|
ILILVY |
47.94 |
47.24 |
-0.70 |
| 5 |
Ali, Anisah M (10)
|
ILFVFY |
55.04 |
47.75 |
-7.29 |
| 6 |
Jordan, Victoria R (10)
|
ILFVFY |
47.35 |
48.01 |
0.66 |
| 7 |
Smith, Taylor M (10)
|
ILFVFY |
50.12 |
49.01 |
-1.11 |
| 8 |
McMurtry, Avelina G (10)
|
ILDCST |
48.29 |
49.07 |
0.78 |
| 9 |
Hall, Olivia G (9)
|
ILDCST |
47.36 |
49.62 |
2.26 |
| 10 |
Rojo, Isabella A (10)
|
ILDCST |
48.50 |
51.13 |
2.63 |
|
Vander Bleek, Samantha G (10)
|
ILDCST |
|
DQ |
- |