| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Torres, Avery (12)
|
GUTCAP |
41.75 |
41.89 |
0.14 |
| 2 |
McManus, Taryn M (12)
|
GUPEAK |
42.75 |
42.04 |
-0.71 |
| 3 |
Delasbour, Tatyana J (11)
|
GUHCAP |
51.99 |
43.99 |
-8.00 |
| 4 |
Stokes, Abby (11)
|
GUTCAP |
40.28 |
45.56 |
5.28 |
| 5 |
Jankowiak, Kyanna (12)
|
GUGIST |
|
45.60 |
- |
| 6 |
Nguyen, Sariel Q (11)
|
GUSCAT |
46.88 |
46.05 |
-0.83 |
| 7 |
Medellin, Mackenzie K (11)
|
GUSSS |
44.17 |
46.30 |
2.13 |
| 8 |
Arreguin, Ayleen (12)
|
GUHCAP |
46.53 |
46.42 |
-0.11 |
| 9 |
Pham, Haleigh T (12)
|
GUSSS |
48.01 |
46.50 |
-1.51 |
| 10 |
Garcia, Ava E (12)
|
GUNOCH |
46.75 |
46.61 |
-0.14 |
| 11 |
Sliger, Madalynn (11)
|
GUTCAP |
44.87 |
46.80 |
1.93 |
| 12 |
Adams, Kaitlin A (11)
|
GUAQUA |
46.44 |
47.22 |
0.78 |
| 13 |
Scanlon, Kassidy (11)
|
GUTCAP |
50.14 |
49.65 |
-0.49 |
| 14 |
Moad, Addyson L (11)
|
GUUN-S |
56.16 |
51.68 |
-4.48 |
| 15 |
Garcia, Alayda I (12)
|
GUNOCH |
51.39 |
52.04 |
0.65 |
| 16 |
Juillerat, Hallie M (11)
|
GUSSS |
1:14.58 |
1:12.12 |
-2.46 |