| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Murray, Miles D (16)
|
NTMTRO |
24.24 |
23.67 |
-0.57 |
| 2 |
Vien, Devlin A (16)
|
NTMTRO |
23.15 |
23.82 |
0.67 |
| 3 |
Torrealba Montilla, Luis D (15)
|
NTMTRO |
24.78 |
24.27 |
-0.51 |
| 4 |
Wang, Brandon M (16)
|
NTMTRO |
24.54 |
25.09 |
0.55 |
| 5 |
Andresen, Maximilian A (17)
|
NTMTRO |
25.52 |
25.44 |
-0.08 |
| 6 |
Sanchez, Jacob R (16)
|
NTMTRO |
25.45 |
25.45 |
0.00 |
| 7 |
Kretiv, Chase W (17)
|
NTMTRO |
24.83 |
25.66 |
0.83 |
| 8 |
Park, Matthew C (15)
|
NTMTRO |
|
26.00 |
- |
| 9 |
Vu, Cole T (17)
|
NTMTRO |
26.40 |
26.29 |
-0.11 |
| 10 |
Huang, Anthony (16)
|
NTMTRO |
26.74 |
26.30 |
-0.44 |
| 11 |
Mayfield, Harper D (18)
|
NTMTRO |
23.64 |
26.50 |
2.86 |
| 12 |
Kozsuch, Kaden L (15)
|
NTMTRO |
24.94 |
26.73 |
1.79 |
| 13 |
Yong, Karston A (16)
|
NTMTRO |
27.16 |
26.95 |
-0.21 |
| 14 |
Bickley, Michael J (15)
|
NTMTRO |
29.43 |
27.24 |
-2.19 |
| 15 |
Samuels, John D (17)
|
NTMTRO |
26.85 |
27.28 |
0.43 |
| 16 |
Howes, Kieffer J (16)
|
NTUN |
28.53 |
28.64 |
0.11 |
| 17 |
Shroff, Ethan A (15)
|
NTMTRO |
|
31.48 |
- |
|
Walenciak, Ross G (17)
|
NTMTRO |
|
NS |
- |
|
Adler, Samuel Y (15)
|
NTMTRO |
27.47 |
NS |
- |