| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Finch, Brooke M (16)
|
NTMTRO |
28.05 |
27.86 |
-0.19 |
| 2 |
Curtis, Devon R (15)
|
NTO2 |
27.61 |
28.08 |
0.47 |
| 3 |
Whitmer, Katherine P (16)
|
NTUN |
28.37 |
28.36 |
-0.01 |
| 4 |
Mather, Hannah E (15)
|
NTSTAR |
28.81 |
28.53 |
-0.28 |
| 5 |
McCormick, Darby J (15)
|
NTSTAR |
29.07 |
28.65 |
-0.42 |
| 6 |
Zevallos, Ava G (15)
|
NTSTAR |
30.23 |
29.36 |
-0.87 |
| 7 |
Welch, Claire A (17)
|
NTMTRO |
27.67 |
29.94 |
2.27 |
| 8 |
Anderson, Payton A (15)
|
NTMTRO |
30.26 |
30.04 |
-0.22 |
| 9 |
Finch, Brinley S (16)
|
NTMTRO |
29.85 |
30.14 |
0.29 |
| 10 |
Mikes, Elana L (15)
|
NTCOPS |
29.46 |
30.72 |
1.26 |
| 11 |
Erwin, Kaylee F (15)
|
NTMTRO |
30.14 |
30.75 |
0.61 |
| 12 |
Bin, Seungyeon (16)
|
NTCOPS |
29.85 |
30.83 |
0.98 |
| 13 |
Boyd, Codie E (16)
|
NTFNW |
31.27 |
31.03 |
-0.24 |
| 14 |
Nguyen, Kayla A (15)
|
NTCOPS |
29.66 |
31.64 |
1.98 |
| 15 |
Jaimes, Alexandria C (15)
|
NTSTAR |
32.78 |
31.74 |
-1.04 |
| 16 |
Perez, Julia G (16)
|
NTSTAR |
|
31.76 |
- |
| 17 |
McKinney, Hannah C (15)
|
NTMTRO |
|
32.65 |
- |
| 18 |
Hebert, Andrea N (16)
|
NTO2 |
|
34.27 |
- |
|
Ohri, Misha M (15)
|
NTMTRO |
29.62 |
NS |
- |
|
Sutherland, Maximillia E (15)
|
NTMTRO |
29.54 |
NS |
- |
|
Hughey, Laurel A (15)
|
NTCOPS |
30.61 |
NS |
- |