| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Monteiro, Phillip G (13)
|
NIVELO |
35.74 |
31.69 |
-4.05 |
| 2 |
Marshall, Parker T (14)
|
NIVELO |
51.74 |
32.38 |
-19.36 |
| 3 |
Murphy, Brian P (14)
|
NITWST |
36.05 |
33.12 |
-2.93 |
| 4 |
Schraufstetter, Derek (14)
|
NIVELO |
43.79 |
34.06 |
-9.73 |
| 5 |
Liu, Daniel (14)
|
NIVELO |
|
34.47 |
- |
| 6 |
Clark, Owen M (14)
|
NITWST |
41.22 |
35.74 |
-5.48 |
| 7 |
Johnson III, James A (14)
|
NIVELO |
59.26 |
36.19 |
-23.07 |
| 8 |
Gaspar, Brandon T (14)
|
NITWST |
38.88 |
37.63 |
-1.25 |
| 9 |
Betterelli, Tristan J (13)
|
NIVELO |
|
41.01 |
- |
| 10 |
Gorney, Caleb A (13)
|
NITTSC |
42.71 |
41.36 |
-1.35 |
| 11 |
Smith, Bradley C (13)
|
NITTSC |
58.50 |
43.75 |
-14.75 |
| 12 |
Sargent, Quinn M (13)
|
NIVELO |
|
45.54 |
- |
| 13 |
Kendall, Jack S (13)
|
NITTSC |
|
50.03 |
- |
|
Drews, Carson M (14)
|
NITWST |
36.34 |
NS |
- |
|
Seifert, Jacob D (13)
|
NITWST |
37.48 |
NS |
- |