| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Roberts, Meredith J (10)
|
NITTSC |
45.38 |
42.54 |
-2.84 |
| 2 |
Rasmusson, Henrietta E (10)
|
NICLSC |
47.01 |
44.36 |
-2.65 |
| 3 |
Malkiewicz, Heather K (10)
|
NITTSC |
48.19 |
45.27 |
-2.92 |
| 4 |
Reilly, Bridget F (10)
|
NISTAR |
47.22 |
46.25 |
-0.97 |
| 5 |
Tunkey, Mila R (10)
|
NIEAST |
50.00 |
48.97 |
-1.03 |
| 6 |
Haacker, Juliana J (10)
|
NITTSC |
|
49.44 |
- |
| 7 |
Baker, Catherine M (9)
|
NITWST |
1:00.33 |
51.80 |
-8.53 |
| 8 |
Dee, Hailey M (9)
|
NISTAR |
1:11.05 |
58.70 |
-12.35 |
| 9 |
Herbert, Reilly M (9)
|
NISTAR |
1:09.41 |
59.72 |
-9.69 |
| 10 |
Washington, Ashanti M (10)
|
NISTAR |
1:00.93 |
1:00.67 |
-0.26 |
| 11 |
Wilson, Sydney N (9)
|
NIBCSR |
|
1:06.78 |
- |
| 12 |
Errington, Julia K (9)
|
NISTAR |
|
1:08.79 |
- |
| 13 |
Ramsey, Yasmin M (9)
|
NITTSC |
1:16.79 |
1:17.55 |
0.76 |
|
Cone, Sadie L (10)
|
NITTSC |
54.23 |
DQ |
- |
|
Bialy, Stella D (10)
|
NISTAR |
1:07.38 |
DQ |
- |
|
Martin, Aaliyah C (10)
|
NIBCSR |
|
DQ |
- |
|
Lawrence, Ella C (9)
|
NITWST |
|
DQ |
- |