| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Yang, Catherine (12)
|
ADUSAM |
39.75 |
40.02 |
0.27 |
| 2 |
Agel, Madison E (12)
|
ADDELM |
40.26 |
41.26 |
1.00 |
| 3 |
Nowitzki, Katherine R (12)
|
ADTAC |
41.99 |
41.32 |
-0.67 |
| 4 |
Olivo-Vukovic, Erika J (11)
|
ADUSAM |
44.04 |
41.89 |
-2.15 |
| 5 |
Schmidt, Christina M (12)
|
ADTAC |
43.62 |
44.09 |
0.47 |
| 6 |
Wyman, Eleanor B (11)
|
ADTAC |
44.53 |
44.12 |
-0.41 |
| 7 |
Cunningham, Isabelle N (12)
|
ADDELM |
45.59 |
44.42 |
-1.17 |
| 8 |
DeLair, Charlize J (11)
|
ADUSAM |
43.98 |
44.53 |
0.55 |
| 9 |
Agro, Lindsay M (11)
|
ADUSAM |
43.78 |
44.70 |
0.92 |
| 10 |
DiPonio, Hannah L (11)
|
ADHAWK |
45.68 |
45.69 |
0.01 |
| 11 |
Santacroce, Payton A (11)
|
ADUSAM |
46.84 |
45.91 |
-0.93 |
| 12 |
Zinnershine, Lily R (12)
|
ADJJSC |
45.20 |
45.94 |
0.74 |
| 13 |
Floyd, Alivia M (12)
|
ADWAVE |
47.05 |
47.18 |
0.13 |
| 14 |
Sliper, Kaylee P (11)
|
ADBSPA |
47.59 |
48.13 |
0.54 |
| 15 |
Oathout, Eve L (11)
|
ADUSAM |
49.51 |
48.52 |
-0.99 |
| 16 |
Banson, Marissa Belle P (11)
|
ADBFIN |
48.64 |
49.77 |
1.13 |
| 17 |
Andreine, Isabelle M (11)
|
ADJJSC |
52.95 |
54.73 |
1.78 |
| 17 |
Pagliuca, Alilynn G (12)
|
ADHAWK |
55.15 |
54.73 |
-0.42 |
| 19 |
Frisone, Gabriella A (11)
|
ADJJSC |
57.19 |
58.93 |
1.74 |
|
Connell, Erin M (11)
|
ADDELM |
|
DQ |
- |
|
DeLisio, Chayse K (11)
|
ADTAC |
50.02 |
NS |
- |
|
Schnitzer, Myalin (12)
|
ADJJSC |
48.58 |
NS |
- |
|
McDonald, Riley B (12)
|
ADJJSC |
55.74 |
NS |
- |
|
Lutz, Olivia G (11)
|
ADJJSC |
48.56 |
NS |
- |