| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Yafayeva, Roxana Z (9)
|
ADDELM |
51.11 |
49.75 |
-1.36 |
| 2 |
Cronlund, Miranda M (9)
|
ADDELM |
50.10 |
50.51 |
0.41 |
| 3 |
Horsch, Jacqueline A (10)
|
ADBSPA |
52.64 |
51.33 |
-1.31 |
| 4 |
Hanchett, Eveline M (9)
|
ADDELM |
56.49 |
53.16 |
-3.33 |
| 5 |
Sliper, Kaylee P (10)
|
ADBSPA |
53.50 |
53.69 |
0.19 |
| 6 |
Fiacco, Briella P (10)
|
ADBFIN |
55.08 |
54.86 |
-0.22 |
| 7 |
Chance, Eleanor M (10)
|
ADGRGF |
1:11.82 |
1:19.17 |
7.35 |
| 8 |
McFarland, Madeline J (9)
|
ADBFIN |
|
1:19.72 |
- |
| 9 |
Triller, Rowan G (9)
|
ADGRGF |
1:14.50 |
1:27.22 |
12.72 |
|
Ahl, Grace E (9)
|
ADBSPA |
57.56 |
DQ |
- |
|
Lundin, Paige R (9)
|
ADBSPA |
1:17.18 |
NS |
- |