| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Becker-Kopke, Taylor (11)
|
AZFORD |
41.94 |
40.52 |
-1.42 |
| 2 |
Kwapich, Ella S (12)
|
AZFAST |
44.92 |
41.28 |
-3.64 |
| 3 |
Figueroa Padilla, Nayla (12)
|
DELS |
48.81 |
42.43 |
-6.38 |
| 4 |
Slater, Athena F (12)
|
BDSWAT |
46.59 |
43.38 |
-3.21 |
| 5 |
Skow, Savannah E (11)
|
NMLCAT |
45.28 |
43.72 |
-1.56 |
| 6 |
Rivera Ruiz, Maria Iveth (11)
|
TIB |
47.11 |
43.98 |
-3.13 |
| 7 |
Quiroz, Taisset (12)
|
MILE |
46.50 |
44.18 |
-2.32 |
| 8 |
Anaya Moreno, Libni Elina (11)
|
CENA |
47.84 |
44.87 |
-2.97 |
| 9 |
Mansell, Marina H (12)
|
AZFORD |
46.04 |
45.75 |
-0.29 |
| 10 |
Slater, Attia F (12)
|
BDSWAT |
41.52 |
45.86 |
4.34 |
| 11 |
Meza, Arissa M (12)
|
BDRAST |
43.77 |
45.97 |
2.20 |
| 12 |
Gallardo Medina, Maria Jose (12)
|
CENA |
45.46 |
46.27 |
0.81 |
| 13 |
Soto-Ruiz, Mikaela R (11)
|
BDRAST |
46.64 |
46.57 |
-0.07 |
| 14 |
Smith, Wren S (11)
|
AZFORD |
41.53 |
47.05 |
5.52 |
| 15 |
Smith, Ruby A (11)
|
AZFORD |
46.30 |
47.25 |
0.95 |