| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lund, Jaycee D (14)
|
SRMVM |
39.04 |
39.29 |
0.25 |
| 2 |
Arnold, Shae L (13)
|
SRNST |
44.99 |
40.18 |
-4.81 |
| 3 |
McHenry, Hannah J (13)
|
SRBSC |
35.12 |
41.27 |
6.15 |
| 4 |
Bytwerk, Zoe R (13)
|
SRBSC |
42.26 |
41.47 |
-0.79 |
| 5 |
Price, Charli J (13)
|
SRBY |
41.14 |
42.16 |
1.02 |
| 6 |
Buschhorn, Jocelyn M (13)
|
SRMVM |
43.07 |
43.71 |
0.64 |
| 7 |
Thomson, Asha L (13)
|
SRMVM |
45.79 |
43.79 |
-2.00 |
| 8 |
Lavering, Anna K (13)
|
SREST |
43.31 |
44.50 |
1.19 |
| 9 |
Gross, Ellie Y (13)
|
SREST |
49.69 |
45.05 |
-4.64 |
| 10 |
Webb, Macy C (14)
|
SRMVM |
50.15 |
48.71 |
-1.44 |
|
Ungerecht, Alexa L (13)
|
SRBY |
43.72 |
DQ |
- |
|
Hansen, Chloe K (13)
|
SRMVM |
51.06 |
NS |
- |
|
Bernard, Madison B (13)
|
SRNST |
53.74 |
NS |
- |
|
Canfield, Berkeley D (13)
|
SRSV5B |
35.75 |
NS |
- |