| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lyons, Ella J (8)
|
MAWAC |
49.89 |
44.84 |
-5.05 |
| 2 |
Lyons, Abigail C (8)
|
MAWAC |
50.28 |
45.24 |
-5.04 |
| 3 |
Devlin, Mia L (8)
|
MAWAC |
|
50.63 |
- |
| 4 |
Ashman, Aubrey V (8)
|
MAWAC |
55.93 |
52.43 |
-3.50 |
| 5 |
Liu, Eden S (8)
|
MAUMAC |
|
52.88 |
- |
| 6 |
Murray, Lily E (8)
|
MABDA |
57.53 |
56.11 |
-1.42 |
| 7 |
Braun, Casey (7)
|
MAUDAC |
1:06.95 |
56.64 |
-10.31 |
| 8 |
Geberth, Rebecca L (8)
|
MATHY |
|
57.90 |
- |
| 9 |
Aiello, Taylor M (7)
|
MAWAC |
|
59.10 |
- |
| 10 |
Krista, Lennon M (7)
|
MALAC |
57.33 |
59.24 |
1.91 |
| 11 |
Persun, Kiley N (8)
|
MAWASC |
|
59.34 |
- |
| 12 |
El Ghissassi, Sidra S (7)
|
NIUN-7 |
56.65 |
59.35 |
2.70 |
| 13 |
Behun, Kathryn E (6)
|
MAUN-1 |
1:07.22 |
1:00.58 |
-6.64 |
| 14 |
Blodgett, Anna J (8)
|
AMSASC |
57.51 |
1:00.82 |
3.31 |
| 15 |
Gulli, Kendall J (7)
|
MAWAC |
|
1:02.21 |
- |
| 16 |
Orr, Aubrie E (8)
|
MAMARA |
|
1:03.45 |
- |
| 17 |
Rivera, Sofia (6)
|
MAT85 |
|
1:07.38 |
- |
| 18 |
Welgosh, Anika R (8)
|
MAUMAC |
|
1:07.59 |
- |
| 19 |
Persun, Kloey E (7)
|
MAWASC |
|
1:09.84 |
- |
| 20 |
Boettger, Rhiley M (6)
|
MAMARA |
|
1:10.62 |
- |
|
Grohsman, Elizabeth M (7)
|
MAUDAC |
|
NS |
- |