| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Park, Jordynn D (14)
|
MAEAC |
24.40 |
25.19 |
0.79 |
| 1 |
Bertolotti, Adriana E (13)
|
MAMSA |
29.75 |
28.65 |
-1.10 |
| 1 |
Bakker, Anna C (14)
|
MAMSA |
|
29.66 |
- |
| 2 |
Lawton, Valerie j (13)
|
MAPENN |
26.65 |
26.79 |
0.14 |
| 2 |
Hoerner, Madison A (14)
|
MAFSC |
29.04 |
29.93 |
0.89 |
| 2 |
Brandwene, Sophie J (13)
|
MANLAC |
|
30.50 |
- |
| 3 |
Cieniuch, Julia (14)
|
MAPENN |
26.89 |
27.22 |
0.33 |
| 3 |
McCloskey, Reilly G (14)
|
MAPENN |
|
30.51 |
- |
| 3 |
Gomez-Fischer, Oriana K (13)
|
MAMSA |
29.88 |
30.95 |
1.07 |
| 4 |
McKim, Paige E (13)
|
MAFSC |
27.93 |
28.35 |
0.42 |
| 4 |
Rutkowski, Lillian G (13)
|
MAEAC |
30.83 |
30.78 |
-0.05 |
| 5 |
Oswald, Abigail C (14)
|
MAMSA |
31.13 |
31.01 |
-0.12 |
| 6 |
Attri, Stuti (13)
|
MAMSA |
33.82 |
31.20 |
-2.62 |
| 7 |
Houck, Sierra G (13)
|
MAEAC |
|
31.57 |
- |
| 8 |
Zhang, Katherine S (13)
|
MAMSA |
33.48 |
31.67 |
-1.81 |
| 9 |
Hamer, Darci (14)
|
MAPENN |
|
33.17 |
- |
| 10 |
Albarano, Margo (13)
|
MAWASC |
33.68 |
33.67 |
-0.01 |
| 11 |
Bhaskar, Shanthi (13)
|
MAMSA |
33.44 |
35.76 |
2.32 |