| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Burns, Abigail J (11)
|
MASOUD |
36.51 |
36.30 |
-0.21 |
| 2 |
Hiestand, Ellie J (11)
|
MASOUD |
37.21 |
36.97 |
-0.24 |
| 3 |
Benson, Margaret A (11)
|
MADST |
37.41 |
37.31 |
-0.10 |
| 4 |
Piccone, Sofia M (11)
|
MAMSA |
39.09 |
37.76 |
-1.33 |
| 5 |
Weaverling, Catherine A (11)
|
MADST |
38.07 |
38.31 |
0.24 |
| 6 |
Curran, Anna N (12)
|
MAPVAC |
40.56 |
38.41 |
-2.15 |
| 7 |
Hinz, Emily M (11)
|
MAGAAC |
40.02 |
38.61 |
-1.41 |
| 8 |
Pike, Kayla M (11)
|
MASOUD |
40.69 |
38.77 |
-1.92 |
| 9 |
Dietrich, Brooke S (12)
|
MAMSA |
38.93 |
38.81 |
-0.12 |
| 10 |
Root, Ella A (11)
|
MAMARA |
38.52 |
39.08 |
0.56 |
| 11 |
Boettcher, Alexandria P (12)
|
MADST |
|
39.09 |
- |
| 12 |
Thomas, Avery L (12)
|
MADST |
40.86 |
39.65 |
-1.21 |
| 13 |
Pistoria, Sydney (12)
|
MAPVAC |
41.43 |
40.05 |
-1.38 |
| 14 |
Bernabeo, Devon E (11)
|
MARAD |
40.84 |
40.92 |
0.08 |
| 15 |
Losco, Riley G (11)
|
MASOUD |
39.40 |
41.01 |
1.61 |
| 16 |
Miller, Lillian (12)
|
MAPVAC |
41.38 |
41.46 |
0.08 |
| 17 |
McGarrity, Hailey (12)
|
MAUN |
42.09 |
41.93 |
-0.16 |
| 18 |
Ransom, Celea M (12)
|
MADST |
44.74 |
42.53 |
-2.21 |
| 19 |
Scheuer, Madison L (11)
|
MAFSS |
44.88 |
42.84 |
-2.04 |
| 20 |
DiSalvo, Lindsay E (12)
|
MAUN |
43.01 |
43.41 |
0.40 |
| 21 |
Slifer, Mildred G (12)
|
MAMSA |
47.39 |
44.90 |
-2.49 |
| 22 |
Moul, Lilly A (12)
|
MAPVAC |
44.64 |
44.97 |
0.33 |
| 23 |
Pruitt, Grace R (11)
|
MADST |
47.90 |
45.30 |
-2.60 |
| 24 |
Hood, Grace D (12)
|
MADST |
49.52 |
45.98 |
-3.54 |
| 25 |
Curcio, Keely B (12)
|
MAPVAC |
49.15 |
46.27 |
-2.88 |
| 26 |
Taylor, Hannah P (11)
|
MAUN |
46.42 |
46.77 |
0.35 |
| 27 |
Reilly, Fina E (11)
|
MAFSS |
49.85 |
48.97 |
-0.88 |
| 28 |
Rigas, Zoe E (12)
|
MADST |
|
49.88 |
- |
| 29 |
Ramirez, Madison T (12)
|
MADST |
|
50.09 |
- |
|
Gonzales, Ana M (11)
|
MAFSS |
59.88 |
NS |
- |
|
Spittle, Amelia G (11)
|
MADST |
44.44 |
NS |
- |