| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
McClain, Kaelyn R (17)
|
AMNAC |
2:40.94 |
2:36.94 |
-4.00 |
| 2 |
Sharar, Ava L (13)
|
AMANSC |
2:47.82 |
2:44.42 |
-3.40 |
| 3 |
Smith, Brooke E (15)
|
AMSFAC |
2:35.16 |
2:45.96 |
10.80 |
| 4 |
King, Abigail (14)
|
AMCSC |
2:35.16 |
2:48.09 |
12.93 |
| 5 |
Meyers, Mackenzie E (14)
|
AMEFSC |
2:42.88 |
2:49.28 |
6.40 |
| 6 |
Lowe, Kate E (14)
|
AMFAST |
2:46.39 |
2:50.83 |
4.44 |
| 7 |
Berg, Madeline M (13)
|
AMSFAC |
2:52.69 |
2:52.20 |
-0.49 |
| 8 |
Toth, Sydney L (13)
|
AMEFSC |
3:04.93 |
2:53.84 |
-11.09 |
| 9 |
Hastings, Megan N (15)
|
AMCSC |
2:45.74 |
2:54.83 |
9.09 |
| 10 |
DeVito, Mia (13)
|
AMCSC |
2:58.00 |
2:56.19 |
-1.81 |
| 11 |
Stratigos, Melina T (13)
|
AMEFSC |
3:03.57 |
2:56.95 |
-6.62 |
| 12 |
Gaydos, Rosemary E (13)
|
AMNAC |
2:52.04 |
2:58.19 |
6.15 |
| 13 |
Walton, Chloe E (13)
|
AMCSC |
3:05.03 |
3:10.48 |
5.45 |
| 14 |
Smith, Kaileigh M (13)
|
AMGPAC |
3:15.20 |
3:13.56 |
-1.64 |
| 15 |
Croushore, Gracie P (14)
|
AMANSC |
3:18.78 |
3:17.62 |
-1.16 |
| 16 |
Abraham, Elizabeth S (14)
|
AMWAAC |
3:23.51 |
3:18.33 |
-5.18 |
| 17 |
Smail, Kendra M (13)
|
AMCSC |
3:16.68 |
3:19.00 |
2.32 |
| 18 |
Hutchison, Isabella N (13)
|
AMCSC |
3:25.67 |
3:21.07 |
-4.60 |
| 19 |
Zanella, Torri R (13)
|
AMWAAC |
3:30.92 |
3:32.76 |
1.84 |
|
Zellers, Jordan M (17)
|
AMCSC |
2:55.68 |
DQ |
- |
|
Hiles, Jennifer A (16)
|
AMGLAC |
2:57.00 |
NS |
- |
|
Baiano, Gabriella L (16)
|
AMSFAC |
2:58.62 |
NS |
- |
|
Smith, Allison M (13)
|
AMBYAC |
3:07.65 |
NS |
- |
|
Mott, Greta K (13)
|
AMANSC |
2:48.78 |
NS |
- |
|
Hanes, Kendall N (14)
|
AMANSC |
3:06.76 |
NS |
- |