| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Van Vreede, Olivia L (14)
|
NIVICT |
56.80 |
56.51 |
-0.29 |
| 2 |
Crye, Joleigh C (14)
|
NIMERC |
|
57.04 |
- |
| 3 |
Pathammavong, Suriyathai A (14)
|
NIVICT |
58.33 |
58.01 |
-0.32 |
| 4 |
Hall, Sofia F (14)
|
NIBAAC |
1:02.28 |
1:01.39 |
-0.89 |
| 5 |
Donlon, Anna M (13)
|
NIWWSC |
1:07.04 |
1:04.28 |
-2.76 |
| 6 |
Newsome, Amber G (13)
|
NIPMSC |
1:17.81 |
1:10.16 |
-7.65 |
| 7 |
Taft, Catrice R (14)
|
NIMERC |
1:06.18 |
1:10.39 |
4.21 |
| 8 |
Barry, Madeline G (13)
|
NIVICT |
1:10.94 |
1:11.70 |
0.76 |
| 9 |
Bugbee, Kyla C (13)
|
NIPMSC |
1:14.17 |
1:12.10 |
-2.07 |
| 10 |
Goebel, Siena J (13)
|
NIISWM |
|
1:13.35 |
- |
| 11 |
Christodaro, Raena M (14)
|
NIHAST |
1:12.95 |
1:13.83 |
0.88 |
| 12 |
Thompson, Daniella J (13)
|
NIGRSC |
1:21.21 |
1:22.99 |
1.78 |
| 13 |
Schicker, Emily M (13)
|
NIGRSC |
|
1:50.96 |
- |